African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5177

Full Length Research Paper

Interaction between bacterial biocontrol-agents and strains of Xanthomonas axonopodis pv. phaseoli effects on biocontrol efficacy of common blight in beans

Bianca Obes Corrêa
  • Bianca Obes Corrêa
  • Master in Agroindustrial Production and Management, Anhanguera – Uniderp University, Zip Code 79037-280 Campo Grande, Brazil.
  • Google Scholar
Vanessa Nogueira Soares
  • Vanessa Nogueira Soares
  • Phytosanitary Department, Agronomy Faculty, Federal University of Pelotas, P. O. Box 354, 96010-970 Pelotas, Brazil
  • Google Scholar
Mauricio Sangiogo
  • Mauricio Sangiogo
  • Phytosanitary Department, Agronomy Faculty, Federal University of Pelotas, P. O. Box 354, 96010-970 Pelotas, Brazil
  • Google Scholar
José Rogério de Oliveira
  • José Rogério de Oliveira
  • Phytopathology Department, Federal University of Viçosa, 36571-000 Viçosa, Brazil.
  • Google Scholar
Andréa Bittencourt Moura
  • Andréa Bittencourt Moura
  • Phytosanitary Department, Agronomy Faculty, Federal University of Pelotas, P. O. Box 354, 96010-970 Pelotas, Brazil
  • Google Scholar


  •  Received: 19 April 2017
  •  Accepted: 07 July 2017
  •  Published: 28 August 2017

Abstract

The bacterial biocontrol agents (BCAs) treatments were used for seed microbiolization; the bacterial biocontrol agents (BCAs) used selected from previous study on the control of Xanthomonas-axonopodis pv. phaseoli Xap) includes: Bacillus (DFs093, DFs348 and DFs769), Pseudomonas (DFs513, DFs831 and DFs842), Rhodococcus (DFs843 and DFs912), and the combinations C01 (DFs093+DFs769+DFs831), C02 (DFs093+DFs769+DFs842) and C03 (DFs093+DFs769+DFs348). Sixteen (16) Xap strains were collected from several Brazilian regions, and were inoculated in cotyledonary leaves. The symptom development was followed for 10 days. The treatments were compared by the area under the disease progress curve for disease incidence, severity, and index. In general, majority of the BCAs reduced, to some degree, the disease caused by different Xap strains.  The combination C01, and the isolate DFs831 resulted in highest mean disease control. The data from this study suggest a relationship between the region of origin of Xap strain and the efficacy of BCA to control the disease caused by it.  The use of combinations of these organisms increased the efficacy of the biocontrol of several strains of the same pathogen. A strain-BCA interaction was shown by data collected from this study, which evidence the importance of selecting a BCA or a combination of BCAs with a wider spectrum of action.

Key words: Biological control, co-inoculation, Bacillus, Pseudomonas, Rhodococcus, bean common blight.