African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4910

Full Length Research Paper

Promising biosurfactant produced by a new Candida tropicalis UCP 1613 strain using substrates from renewable-resources

Daylin Rubio-Ribeaux
  • Daylin Rubio-Ribeaux
  • Post-graduation Program in Biological Sciences, Federal University of Pernambuco, Brazil 50670-420, Recife, Pernambuco, Brazil.
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Rosileide Fontenele da Silva Andrade
  • Rosileide Fontenele da Silva Andrade
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, Brazil 50050-590, Recife, Pernambuco, Brazil.
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Goretti Sonia da Silva
  • Goretti Sonia da Silva
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, Brazil 50050-590, Recife, Pernambuco, Brazil.
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Rodrigo Assuncao de Holanda
  • Rodrigo Assuncao de Holanda
  • CEUMA University, São Luís, 65075120, Maranhão, Brazil.
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Milagre Americo Pele
  • Milagre Americo Pele
  • Post-graduation Program in Biological Sciences, Federal University of Pernambuco, Brazil 50670-420, Recife, Pernambuco, Brazil.
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Patricia Nunes
  • Patricia Nunes
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, Brazil 50050-590, Recife, Pernambuco, Brazil.
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Jose Carlos Vilar Junior
  • Jose Carlos Vilar Junior
  • Northeast Network for Biotechnology, Federal Rural University of Pernambuco, 52171-900 Recife, PE, Brazil.
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Maria Aparecida de Resende-Stoianoff
  • Maria Aparecida de Resende-Stoianoff
  • Institute for Biological Sciences, Federal University of Minas Gerais, 31270-010, Belo Horizonte, Minas Gerais, Brazil.
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G. M. Campos-Takaki
  • G. M. Campos-Takaki
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, Brazil 50050-590, Recife, Pernambuco, Brazil.
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  •  Received: 15 February 2017
  •  Accepted: 26 May 2017
  •  Published: 21 June 2017

Abstract

The current work aimed to use agro-industrial wastes as a strategy to obtain biosurfactant from a new Candida tropicalis UCP 1613 isolated from mangrove sediments of Rio Formoso, Pernambuco State, Brazil. The yeast was identified based on 18S rRNA sequencing method and blast homology search. The promising strain showed the ability to use an optimal composition of a cheap medium containing whey (3%), cassava wastewater (7%) and soybean post frying oil (10%) during 96h which reduced significantly the surface tension from 70 to 28.8 mN/m. The yield of the biosurfactant obtained was 4.9 g/L and the minimum value of critical micelle concentration was 1.5%. In addition, this was isolated and characterized as an anionic polymeric molecule, composed of proteins (51%), lipids (37%) and carbohydrates (11%), and confirmed by Fourier transform infrared spectroscopy (FTIR) analysis. Also, biosurfactant was capable in forming stable emulsions at different ranges of temperature, pH and salinity. Alternatively, the biosurfactant displayed no toxicity against the different vegetable evaluated seeds Brassica oleracea, Lactuca sativa L. and Solanum lycopersicum. The antimicrobial activity of this biosurfactant was verified showing the main efficacy against Gram-positive bacteria besides inhibiting the antifungal activity against the yeast tested. The results obtained suggest its potential application to pharmaceutical, as well as environmental area.

 

Key words: Candida tropicalis, mangrove sediment, oil dispersion, biosurfactant.