African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5228

Full Length Research Paper

Characterization of a Shiga toxin 2-encoding bacteriophage ФMin27 isolated from Escherichia coli O157:H7 Strain of China

Liangke Su1, Chengping Lu1,2, Luming Xia3, Yan Wang1, Jian Wang3, Jianhe Sun1 and Yaxian Yan*1
1Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. 2Key Laboratory of Animal Disease Diagnosis and Immunology of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095,China. 3Shanghai Center for Animal Disease Control and Prevention, Shanghai 201103, China.  
Email: [email protected]

  •  Accepted: 19 October 2009
  •  Published: 30 November 2009

Abstract

A Shiga toxin 2 (Stx2) -encoding bacteriophage ФMin27, isolated from Escherichia coli O157:H7 strain of China, having a regular hexagonal head and short tail, was integrated into the host wrbA gene and sequenced with a length of 63,395 nucleotides. ФMin27 had a high homology compared to reported Stx2 phage 933W, while three genes (O, P and ORF31) in the replication region were homologous to genes of phage Shigella flexneri V (SFV) of S. flexneri 5 strain 8401 isolated in China. It demonstrated that ФMin27 was closely related to phage 933W though they were isolated from different geographic areas, but an obvious regionally genetic exchange had occurred in its replication region.

 

Key words: Stx2 bacteriophage, ФMin27, phage micrograph, integration site, phage genome.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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