African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Production and purification of polyclonal antibodies against 34 kDa protein (P34) of Mycobacterium avium subsp. Paratuberculosis

Hafezeh Alizadeh
  • Hafezeh Alizadeh
  • Department of Biology, Science & Research Branch, Islamic Azad University, Tehran-Iran
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Rasool Madani*
  • Rasool Madani*
  • Department of Biochemistry and Proteomics, Razi Vaccine and Serum Research Institute, Karaj-Iran
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Narges Kavid
  • Narges Kavid
  • Department of Biochemistry and Proteomics, Razi Vaccine and Serum Research Institute, Karaj-Iran
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Fariba Golchinfar
  • Fariba Golchinfar
  • Department of Biochemistry and Proteomics, Razi Vaccine and Serum Research Institute, Karaj-Iran
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Tara Emami
  • Tara Emami
  • Department of Biochemistry and Proteomics, Razi Vaccine and Serum Research Institute, Karaj-Iran
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  •  Received: 27 December 2013
  •  Accepted: 12 May 2014
  •  Published: 14 May 2014

Abstract

Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis in ruminants. Among molecular components of MAP, protein P34 was identified as specific and immunodominant. Here, we describe the production of polyclonal antibodies with defined specificity for P34. Polyclonal antibodies were generated from New Zealand white rabbit. Animals were immunized at a certain time period with purified P34, MAP antigens and Freund’s adjuvant. Antibodies were purified from serum by ion exchange chromatography. Western blotting analysis was used for evaluation of interaction between 34 kDa protein and antibodies.

Key words: 34 kDa antigen, Paratuberculosis, Polyclonal antibodies.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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