Full Length Research Paper
Abstract
Phosphorylcholine (PC) has been reported to help PC-bearing Aggregatibacter actinomycetemcomitans to gain access to the general circulation via interaction with the platelet-activating factor receptor (PAFR) on endothelial cells. However, the underlying mechanism remains unclear. Here, we found that inhibition of clathrin and β-arrestins blocked bacterial PC binding to PAFR which signiï¬cantly attenuated A. actinomycetemcomitans invasion of human vascular endothelial cells (HUVEC). These results demonstrated the involvement of clathrin and β-arrestins in PC-bearing A. actinomycetemcomitans invasion of HUVEC.
Key words: Aggregatibacter actinomycetemcomitans, clathrin, β-arrestins, human vascular endothelial cells.
Abbreviation
PC, Phosphorylcholine; PAFR, platelet-activating factor receptor; HUVEC, human vascular endothelial cells; GPCRs, G proteins coupled receptors; MDC, monodansylcadaverine; CPZ, chlorpromazine; MOI, multiplicity of infection
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