Abstract

Characterization of seven Xanthomonas strains comprising four species was done using molecular techniques, that is, 16S rRNA gene amplification and SDS-PAGE analysis. 16S rRNA gene amplification was performed to confirm the identity of strains. The primer sets used in the present study amplified 0.352 Kb and 1.3 Kb sized fragments of rRNA gene in all the strains. The species of the genus Xanthomonas exhibited relatively high levels of overall similarity. SDS-PAGE analysis was done to check the protein profile of the isolates and homogeneous banding pattern was observed that confirmed the authenticity of strains as Xanthomonas. A database of SDS-protein patterns provides a valuable tool for the identification of unknown Xanthomonas.

Key words: Xanthomonas, 16S rRNA gene amplification, SDS PAGE