African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5096

Table of Content: 23 April, 2012; 6(15)

April 2012

Diversity of NosZ gene in three municipal wastewater treatment plants located in different geographic regions

Diversity of nitrous oxide reductase genes (NosZ) in activated sludge was compared in the biological treatment process (BTCs, anaerobic, anoxic and aerobic process) of three municipal wastewater treatment plants located in different geographic regions. A total of 18 specific bands generated after polymerase chain reaction- denaturing gradient gel electrophoresis (PCR-DGGE) analysis. The diversity of NosZ in MWTPs of...

Author(s): Jun Hou, Li Li, Songhe Zhang, Peifang Wang and Chao Wang

April 2012

New insight into the regulation of classâ…¡ bacteriocin production by quorum sensing in Lactobacillus pentosus 31-1

The biosynthesis of class II bacteriocins is frequently regulated by a quorum-sensing regulatory mechanism meditated by autoinducing peptide. Here, it was preliminarily confirmed that bacteriocin production by Lactobacillus pentosus 31-1 was controlled by a quorum sensing (QS) system meditated by peptide signal molecules in the cell-free supernatant (CFS). Effects of the growth medium and inoculums size...

Author(s): Xiangmei Zhang, Guorong Liu, Bin Zhao and Pinglan Li 

April 2012

Biological potential of Pseudomonas sp. BS1 in the control of Phytophthora root rot of soybean

In this study, the biological potential of Pseudomonas sp. BS1 in the control ofPhytophthora root rot of soybean was investigated. Fermentation filtrates ofPseudomonas sp. BS1 inhibited zoosporangium formation and zoospore germination ofPhytophthora sojae (P. sojae), and the inhibitory effect gradually weakened with the increasing filtrate dilution. It had a relatively strong...

Author(s): Jumei Hou Sining Bi, Lei Yan, Yuhu Zuo, Yanjie Wang, Tong Liu and Jiewei Zhu

April 2012

Isolation of lectin and lectin derivatives from Haworthia limifolia: An in-vitro investigation

The ability for medicinal plants to aid in wound healing lends itself to cell-agglutinating and sugar-specific protein. These are collectively termed lectins. Although, the function of these lectins in plants are limiting, the benefit to human in terms of wound healing is of vital importance. With many new infections arising as a result of bacteria, fungi and viruses developing immunity against western medicines, the...

Author(s): R. M. Coopoosamy and K. K. Naidoo

April 2012

Iranian traditional medicine: Comparison of the antibacterial effect of ANNAS 0.2% and chlorhexidine 0.2%

Anbarnesa smoke have a long history of antimicrobial effects in Iranian traditional medicine. The present study compared the effect of ANNAS (extract of Anbarnesara smoke) mouth wash 0.2% and Chlorhexidine 0.2% against some bacterial species. In this experimental study, the specific culture mediums were used for each species and the antibacterial efficacies of the mouth rinses were determined by means of agar diffusion...

Author(s): Hasanali Shafiee, Hamed Mortazavi, Maryam Baharvand, Guita Eslami, Sedigheh Bakhtiari, Soodabeh Taheri, Fatemeh Namazi, Amin Asgari, Somayyeh Azimi and Khojasteh Joharchi

April 2012

Optimization of the culture conditions of a Chlamydomonas high oil content ultraviolet mutant CC124-M25 and polymorphism analysis by inter-simple sequence repeat (ISSR)

Microalgal oils have been considered as a promising feedstock for biodiesel production, which can potentially completely substitute fossil fuels. In the present study, 4500 mutants were obtained from Chlamydomonas reinhardtii CC124 through ultraviolet (UV) induced mutagenesis. After screening these mutants using HSM and HSM-N media, a mutant CC124-M25 with high oil content was selected under an HSM-N medium...

Author(s): Xiaodong Deng, Yujiao Zhou, Yajun Li, and Xiaowen Fei,  

April 2012

In vitro synergy profile of some antibiotics against mono and multidrug resistant clinical isolates of Pseudomonas aeruginosa

It is becoming highly challenging for scientific community around the world to battle against rapidly increasing deadly superbugs. Because of heavy exposure of antibiotics and inadequate regimen against infectious organisms, antibiotic resistance in these pathogens has created an immense problem worldwide. Combined antibiotic therapy is frequently recommended for the treatment of such problems. This study is focused to...

Author(s): Zafar Ahmed, Shaukat Saeed Khan and Mahnaaz Khan

April 2012

Influence of substrate concentration in accumulation pattern of poly(R) hydroxyalkonoate in Pseudomonas putida SU-8

Polyhydroxyalkanoates (PHAs) are accumulated by many bacteria intracellularly as carbon and energy reserves in presence of excess carbon source and limited nitrogen or other nutrient sources. The accumulation pattern of these PHA may vary as the supplements are varied. In this study, Pseudomonas putida SU-8 was allowed to grow in glucose and lactose containing medium, the organism was found to accumulate more...

Author(s): Jawahar Nisha, Nithya Mudaliar, Senthilkumar P., Narendrakumar and Antony V. Samrot

April 2012

Prevalence of Hepatitis B detected through RT PCR in Khyber Pakhtunkhwa

Pakistan is highly endemic for Hepatitis B virus. Many studies have shown that 32% of patients with liver diseases are suffering with hepatitis B. Objective: The present project was designed to check the prevalence of Hepatitis B in those peoples of Khyber Pakhtunkhwa who visited a specific laboratory for diagnosis. The method included a four months data of Hepatitis B positive patients from January 2011 to April 2011,...

Author(s): Department of Bioinformatics and Biotechnology International Islamic University, Islamabad, Pakistan. Department of Biotechnology, University of Malakand, Chakdara, Lower Dir, Pakistan. National Reference Laboratory for Tuberculosis, NIH, Islamabad, Pakistan. Department of Microbiology, Hazara University, Mansehra, KPK, Pakistan

April 2012

Comparison of two mechanical instrumentation techniques in reducing root canal bacterial population

Microorganisms and their products play an important role in the pathogenesis of pulpal and periradicular diseases, so their elimination from root canal system as well as reinfection prevention are the main purpose of root canal treatment. This study was designed to compare the in-vitro efficiency of the Hero and M-Two rotary instruments in intracanal bacterial reduction. Fifty six human extracted teeth were...

Author(s): Mahsa Eskandarinezhad, Hooman Sadrhaghighi, Mona Rahmani, Nima Moradi Majd and Alireza Sadighi

April 2012

Comparative study on decolorization of reactive dye 222 by white rot fungi Pleurotus ostreatus IBL-02 and Phanerochaete chrysosporium IBL-03

White rot basidiomycetes have unique ability to decolorize synthetic industrial dyes. Initial experiment was performed with five locally isolated indigenous white rot species fungi S.commune IBL-01 (SC), P. ostreatus IBL-02 (PO) P. chrysosporium IBL-03 (PC), T.versicolor IBL-04 (TV) and G. lucidum IBL-05 (GL), for the selection of white rot fungal cultures...

Author(s): Shumaila Kiran, Shaukat Ali, M. Asgher and Farooq Anwar

April 2012

Identification of indigenous Xanthomonas isolates through 16S rRNA gene amplification and SDS PAGE analyses

Characterization of seven Xanthomonas strains comprising four species was done using molecular techniques, that is, 16S rRNA gene amplification and SDS-PAGE analysis. 16S rRNA gene amplification was performed to confirm the identity of strains. The primer sets used in the present study amplified 0.352 Kb and 1.3 Kb sized fragments of rRNA gene in all the strains. The species of the...

Author(s): Sabin Fatima, Tehmina Anjum, Rukhsana Bajwa and Ahmad Ali Shahid  

April 2012

Ecological studies on nitrogen fixing bacteria from leguminous plants at the north of Jordan

The rates of nitrogen fixation by symbiotic rhizobia are often two to three times higher than the ones exhibited by free-living nitrogen-fixing bacteria in soil. In this study, Rhizobium leguminosarum, Rhizobium meliloti, Rhizobium loti and Rhizobium ciceri were isolated and identified from leguminous plants grown at the north of Jordan. The aim of this study was initially to determine the...

Author(s): Abu-Zaitoon Yousef and Sallal Abdul-Karim  

April 2012

Detection of Salmonella, Clostridium perfringens and Escherichia coli from fecal samples of captive animals at the National Zoological Gardens of South Africa

Escherichia coli, Clostridium perfringens and Salmonella spp. are enterotoxic, ubiquitously distributed bacteria. Diseases caused by these pathogens are well recognized in humans, livestock, companion and zoo animals and can result in morbidity, mortality, and significant economic losses. The aim of the study was to identify and simultaneously amplify enterotoxigenic E....

Author(s): Khethiwe Mtshali, Moses S. Mtshali, Jane S. Nkhebenyane and Oriel M. M. Thekisoe

April 2012

Evaluation of antibacterial properties and biochemical effects of monoterpenes on plant pathogenic bacteria

The antibacterial activity of twelve monoterpenes, namely camphene, (R)-camphor, (R)-carvone, 1,8-cineole, cuminaldehyde, (S)-fenchone, geraniol, (S)-limonene, (R)-linalool, (1R,2S,5R)-menthol, myrcene and thymol was tested against two plant pathogenic bacteria Agrobacterium tumefaciens and Erwinia carotovora var. carotovora using agar dilution method. For a better understanding...

Author(s): Mona A. Abdel Rasoul, Gehan I. Kh. Marei and Samir A. M. Abdelgaleil  

April 2012

The T3S effector XopXccN of Xanthomonas campestris pv. campestris is involved in plant defense through interference with photosystems, reactive oxygen species (ROS) generation, and callose deposition

XopXccN and XopN are important type III secreted (T3S) virulence effector proteins ofXanthomonas campestris pv. campestris (Xcc) and Xanthomonas campestris pv.vesicatoria (Xcv), respectively. They were placed in the same T3S effector class based on sequence homology. It has been demonstrated that Xcv XopN suppresses pathogen-associated molecular pattern-triggered immune responses...

Author(s): Guo-Feng Jiang, Bo-Le Jiang, Li-Gang Chen, San Liu, Hong-Yu Wei, Wei-Jian Cen, Xiao-Hong Hang, Zong-Zhen Wen, Dong-Jie Tang, Guang-Tao Lu, Yong-Qiang He, Di-Qiu Yu and Ji-Liang Tang

April 2012

Antimicrobial effect of amphotericin B electronically-activated water against Candida albicans

The activation of water by physical means stimulates a new scientific approach to microbiology, in particular, antimicrobial methods. However, many of these methods are unproven or have not been properly tested. Since the 1980s, a promising procedure known as biophysical-information therapy or bioresonance therapy (BRT) has emerged as an alternative method against microbial diseases, but it has not yet been properly...

Author(s): J. Antonio Heredia-Rojas, Ricardo Gomez-Flores, Abraham O. Rodríguez-de la Fuente, Enriqueta Monreal-Cuevas, Antonio Cayetano Torres-Flores, Laura E. Rodríguez-Flores, Michaela Beltcheva and Antonio Cayetano Torres-Pantoja  

April 2012

Inhibitory influence of biocontrol agents, plant oils, and an inorganic salt on Mycosphaerella fijiensis and Cordana musae

The search for alternatives to chemical treatments to control the causal pathogens of black sigatoka and Cordana leaf spot in banana was studied in vitro. We examined the effect of biocontrol agents, plant oils, and an inorganic salt on the mycelium growth ofMycosphaerella fijiensis and Cordana musae for 10 weeks. Trichoderma harzianumDGA01 and Bacillus...

Author(s): Dionisio G. Alvindia

April 2012

Susceptibility pattern among pulmonary and extrapulmonary isolates of Mycobacterium tuberculosis in north India

Drug resistant tuberculosis (TB) is an increasing problem worldwide. We retrospectively identified Mycobacterium tuberculosis isolates from treatment failure pulmonary TB (362),and extrapulmonary TB (338) patients (18-70 years) during a 4 year period (June, 2002-July, 2006). Drug susceptibility testing was carried out for the first line anti-tuberculosis drugs using standard proportion method. Of the...

Author(s): Sunil Sethi, Manisha Biswal, Shiv Shekhar Chatterjee, Abhishek Mewara, Dheeraj Gupta, Shiv Kumar, Khushwinder Singh and Meera Sharma

April 2012

Screening of rhizobacteria against Fusarium oxysporum f. sp. melongenae, the causal agent of wilt disease of eggplant

Rhizosphere isolates of Bacillus and Pseudomonas species isolated from the roots of eggplants (Solanum melongena L.) were collected in eggplant cultivated areas in various geographical districts of Turkey. Isolates were evaluated for siderophore, protease and cyanide production along with the cell wall degrading enzyme production as well as phosphate solubilizing and nitrogen fixing...

Author(s): H. N. Yildiz, H. Handan Altinok, Murat Dikilitas

April 2012

The effects of aluminum stress on bacterial community diversity in acidic red soils by polymerase chain reaction (PCR)-amplified restriction fragment length polymorphism

The soil bacterial communities in response to aluminum (Al) treatments were evaluated by polymerase chain reaction-amplified restriction fragment length polymorphism (PCR-RFLP) of 16S rRNA genes in acidic red soil samples in this study. A total of 6 bacterial communities were sampled from two representative soil types under different Al-treated concentrations. Bacterial genomic DNA was extracted and nested PCR-amplified...

Author(s): Genhe He, Junyue Lin, Qiang Liu, Jingfei Zhang and Jichun Wu