Abstract

Two groups of the extracts from different Gastrodia elata BL.s were separately added into the fermentation system of Grifola frondosa in submerged culture. The results showed that the two extracts could promote the biomass growth of Grifola frondosa significantly (P<0.05). However, the extracts of Gastrodia elata BL. in the group 2 would contribute more  to Grifola frondosa’s biomass growth than that in the group 1, and the extracts of Gastrodia elata BL. in the group 2 containing 5% (v/v) markedly promoted the biomass and extracellular polysaccharide (EPS) biosynthesis of Grifola frondosa (P<0.05). Biomass and EPS production increased from 0.564±0.09 to 1.324±0.25 g/l and from 71.69±0.53 to 107.08±0.85 mg/l, which increased by 134.75 and 49.37%, respectively. However, intracellular polysaccharides (IPS) content declined from 60.38±0.87 to 45.71±0.66 mg/g, which decreased by 24.30% compared with the control’s, respectively. Moreover, a fact showed that EPS-2 and IPS-2 were both the main components, and there were no new other polysaccharide components separated by DEAE-52 column in the sample. It suggested that the polysaccharide biosynthesis pathways of Grifola frondosa may be unchanged.

Key words: Grifola frondosa, Gastrodia elata BL., extract, extracellular polysaccharide (EPS), intracellular polysaccharides (IPS).