African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5124

Full Length Research Paper

Prevalence and characterization of extended-spectrum ?-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from poultry in Ouagadougou, Burkina Faso

Souleymane Soré
  • Souleymane Soré
  • Medical Biology Laboratories, Health Ministry, P. O. Box 7022, Ouagadougou 03, Burkina Faso.
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Fatimata B. Josiane Diarra
  • Fatimata B. Josiane Diarra
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food (LaBESTA), Center for Research in Biological, Food and Nutritional Sciences (CRSBAN), Graduate School of Science and Technology (EDST), University of Ouaga I Professor Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina Faso.
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Juste Isidore Bonkoungou
  • Juste Isidore Bonkoungou
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food (LaBESTA), Center for Research in Biological, Food and Nutritional Sciences (CRSBAN), Graduate School of Science and Technology (EDST), University of Ouaga I Professor Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina Faso.
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Charles Sawadogo
  • Charles Sawadogo
  • Medical Biology Laboratories, Health Ministry, P. O. Box 7022, Ouagadougou 03, Burkina Faso.
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Boubié G. Bationo
  • Boubié G. Bationo
  • Medical Biology Laboratories, Health Ministry, P. O. Box 7022, Ouagadougou 03, Burkina Faso.
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Hervé Ky
  • Hervé Ky
  • Medical Biology Laboratories, Health Ministry, P. O. Box 7022, Ouagadougou 03, Burkina Faso.
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Patrick Djim-Madjim Madingar
  • Patrick Djim-Madjim Madingar
  • Medical Biology Laboratories, Health Ministry, P. O. Box 7022, Ouagadougou 03, Burkina Faso.
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Abdoul Salam Ouédraogo
  • Abdoul Salam Ouédraogo
  • Souro Sanou University Hospital, Bobo-Dioulasso, Burkina Faso.
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Idrissa Sanou
  • Idrissa Sanou
  • School of Sciences, Health and Technologies, Saint Thomas d´Aquin University, P. O. Box 10212, Ouagadougou 06, Burkina Faso.
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  •  Received: 20 January 2021
  •  Accepted: 12 March 2021
  •  Published: 31 August 2021

Abstract

This study aimed to determine the prevalence of extended spectrum beta-lactamase producing (ESBL) and multidrug resistant Escherichia coli and Klebsiella pneumoniae. A cross-sectional study was conducted in Ouagadougou in two poultry farms and two slaughterhouses. 375 cloacal swabs and 46 environment samples were collected and members of Enterobacteriaceae were isolated on EMB agar containing 4 µg/L of cefotaxime. E. coli and K. pneumoniae were identified using biochemical tests and ESBL production was confirmed by the double-disc synergy test. Antibiotic susceptibility was determined by the disc diffusion method. Prevalence of faecal ESBL producing E. coli and K. pneumoniae was 12.11% (95% CI = 9.3-15.6). In sampling sites, the prevalence were 5.15% in Farm A, 2.22% in Farm B, 17.50% in slaughterhouse C, 20.59% in slaughterhouse D and 19.57% in environment. E. coli (n = 43) and K. pneumoniae (n = 13) were frequently identified. ESBL-producing E. coli and K. pneumoniae MDR was 89.29% (95% CI = 78.5–95.0). Resistance to aminoglycosides was 6.25% in poultry and 10.00% in slaughterhouse, fluoroquinolones 32.5% in slaughterhouse, sulfonamides 100% in poultry and 82.50% slaughterhouse, tetracycline 100% in poultry and 95.0% in slaughterhouse. This study showed that antimicrobial resistance in poultry in Ouagadougou portends a serious problem.

 

Key words: Escherichia coli, Klebsiella pneumoniae, extended-spectrum β-lactamase (ESBL), poultry, farms, slaughterhouses, Ouagadougou.