African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5182

Full Length Research Paper

Emergence of Diarrhoeagenic Klebsiella pneumoniae Carrying astA and senB genes in Nigeria

David Olusoga Ogbolu
  • David Olusoga Ogbolu
  • Department of Medial Laboratory Science, College of Health Sciences, Osun State University, P.M.B. 4494 Nigeria.
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Akinwale Majeed Akinlabi
  • Akinwale Majeed Akinlabi
  • Department of Medical Microbiology and Parasitology, College of Health Sciences, Olabisi Onabanjo University, Ago-Iwoye, Nigeria.
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Adeolu Sunday Oluremi
  • Adeolu Sunday Oluremi
  • Department of Medial Laboratory Science, College of Health Sciences, Osun State University, P.M.B. 4494 Nigeria.
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Adewale Adegboyega Oke
  • Adewale Adegboyega Oke
  • Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, Ajayi Crowther University, Oyo, Nigeria.
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Mark Alexander Webber
  • Mark Alexander Webber
  • Microbes in the Food Chain, Quadram Institute Bioscience, Norwich Research Park, Norwich, United Kingdom, NR4 7UQ, UK.
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  •  Received: 18 January 2022
  •  Accepted: 31 May 2022
  •  Published: 31 July 2022

Abstract

Klebsiella pneumoniae is increasingly being isolated from the stool of Nigerian patients. K. pneumoniae was isolated from stool samples submitted to two clinical laboratories in Nigeria from patients presenting with various symptoms of gastrointestinal tract infections, including diarrhoea. The authors characterized the virulence and antimicrobial resistance genes of these K. pneumoniae strains. Sixteen pure cultures of heavy growth of K. pneumoniae isolated from two facilities in Nigeria were subjected to susceptibility testing using a panel of antibiotic, with agar dilution method. Paired-end Illumina whole genome sequencing was completed using a NextSeq instrument. Virulence genes including astA, senB, and gad were found in 5 isolates. Multiple plasmid replicons were present; IncF and Col were common plasmids while others were IncR and IncY. Four different STs were found; ST914, ST1962, ST494, and novel ST. These isolates carried various important resistance genes to cephalosporins, fluoroquinolones, aminoglycosides, and so on, including blaCTX-M-15 in one of the isolates. Diarrhoeagenic K. pneumoniae is present, which is caused by plasmid-mediated virulence genes such as astA, senB, and gad. Fluoroquinolone and third generation cephalosporin resistance were discovered.

 

Key words: Klebsiella, diarrhoea, virulence genes, antibiotics resistance, genomics, Nigeria.