African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4881

Full Length Research Paper

OXA-48 type carbapenemase in Klebsiella pneumoniae producing extended spectrum B-lactamases (ESBL) in Senegal

Lo Seynabou
  • Lo Seynabou
  • UFR des Sciences de la Santé, Université Gaston Berger, BP 234, Saint Louis, Sénégal.
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Robin Frédéric
  • Robin Frédéric
  • CNR de la Résistance des Entérobactéries, CHU Gabriel Montpied, Clermont Ferrand, France.
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Beyrouthy Racha
  • Beyrouthy Racha
  • CNR de la Résistance des Entérobactéries, CHU Gabriel Montpied, Clermont Ferrand, France.
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Ba-Diallo Awa
  • Ba-Diallo Awa
  • Faculté de Médecine, Pharmacie et Odonto-Stomatologie, UCAD, Dakar, Sénégal.
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Niang Aïssatou Ahmet
  • Niang Aïssatou Ahmet
  • Faculté de Médecine, Pharmacie et Odonto-Stomatologie, UCAD, Dakar, Sénégal.
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Diagne Rokhaya
  • Diagne Rokhaya
  • UFR des Sciences de la Santé, Université de Thiès, Sénégal.
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Diop Amadou
  • Diop Amadou
  • Faculté de Médecine, Pharmacie et Odonto-Stomatologie, UCAD, Dakar, Sénégal.
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Camara Makhtar
  • Camara Makhtar
  • Faculté de Médecine, Pharmacie et Odonto-Stomatologie, UCAD, Dakar, Sénégal.
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Ka Roughyatou
  • Ka Roughyatou
  • UFR des Sciences de la Santé, Université de Thiès, Sénégal.
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Gaye-Diallo Aïssatou
  • Gaye-Diallo Aïssatou
  • Faculté de Médecine, Pharmacie et Odonto-Stomatologie, UCAD, Dakar, Sénégal.
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Sow Ahmad Iyane
  • Sow Ahmad Iyane
  • Faculté de Médecine, Pharmacie et Odonto-Stomatologie, UCAD, Dakar, Sénégal.
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Bonnet Richard
  • Bonnet Richard
  • CNR de la Résistance des Entérobactéries, CHU Gabriel Montpied, Clermont Ferrand, France.
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  •  Received: 06 March 2018
  •  Accepted: 07 May 2018
  •  Published: 14 May 2018

Abstract

Enterobactericeae producing extended spectrum b-lactamases (ESBL) are likely to express carbapenemases OXA-48 which have hydrolytic activity on carbapenems. The aim of this work was to evaluate prevalence of blaOXA-48 for Klebsiella pneumoniae isolates producing ESBL. This work was conducted at the French Reference Center for Antibiotic Resistance with strains from Senegalese Hospital. Standard antibiogram was performed in accordance to CA-SFM/EUCAST 2016 and presence of ESBL was confirmed by synergistic image. Polymerase chain reaction (PCR) was performed to detect systematically blaOXA-1, blaTEM-1, blaCTX-M-1, blaCTX-M-9 and blaOXA-48 gene in case of decrease sensitivity to carbapenem. PCR products were extracted, purified, sequenced and whole-genome sequence (WGS) were used for the analyses. Plasmids extraction was performed by Kado and Liu method. Five isolates harbored a decreased susceptibility to carbapenems. They were positive for blaOXA-48 gene and also expressed blaCTX-M-15. Analysis of the five plasmids by WGS identified a single IncL/M type plasmid of 63 kb and other genes for aminoglycosides and quinolones resistance. Carbapenemase-producing Enterobacteriaceae represent new threat to public health. Decrease in carbapenem susceptibility should be an alert for rapid detection of carbapenemases and to prevent their spread. Phenotypic or molecular methods should be available in many laboratories to take appropriate preventive and therapeutic measures. 

Key words: Klebsiella pneumoniae, extended spectrum b-lactamases (ESBL), carbapenemase, blaOXA-48, Senegal.