African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4881

Full Length Research Paper

Determination of aflatoxin M1 in bovine milk from the Alagoas/Brazil State dairy belt by high performance liquid chromatography (HPLC)

Angela Matilde da Silva Alves
  • Angela Matilde da Silva Alves
  • Instituto Federal de Educação, Ciência e Tecnologia de Alagoas/Brasil.
  • Google Scholar
Pabllo Henrique de Souza Lima
  • Pabllo Henrique de Souza Lima
  • Instituto Federal de Educação, Ciência e Tecnologia de Alagoas/Brasil.
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Mario Alberto Santos da Costa
  • Mario Alberto Santos da Costa
  • Instituto Federal de Educação, Ciência e Tecnologia de Alagoas/Brasil.
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Cristian Bernardo da Silva
  • Cristian Bernardo da Silva
  • Instituto Federal de Educação, Ciência e Tecnologia de Alagoas/Brasil.
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Jonas dos Santos Sousa
  • Jonas dos Santos Sousa
  • Instituto Federal de Educação, Ciência e Tecnologia de Alagoas/Brasil.
  • Google Scholar
Danielle dos Santos Tavares Pereira
  • Danielle dos Santos Tavares Pereira
  • Instituto Federal de Educação, Ciência e Tecnologia de Alagoas/Brasil.
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Phabyanno Rodrigues Lima
  • Phabyanno Rodrigues Lima
  • Instituto Federal de Educação, Ciência e Tecnologia de Alagoas/Brasil.
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Johnnatan Duarte de Freitas
  • Johnnatan Duarte de Freitas
  • Instituto Federal de Educação, Ciência e Tecnologia de Alagoas/Brasil.
  • Google Scholar


  •  Received: 15 March 2018
  •  Accepted: 28 June 2018
  •  Published: 07 July 2018

Abstract

Milk is considered a nutritionally noble food and is therefore suitable for feeding children and adults. However, contamination of milk by mycotoxins may pose a health risk to the consumer. Aflatoxins, mycotoxins produced by fungi of the genus Aspergillus, can be found in several food products, including milk and its derivatives, which reinforces the importance of this type of study on the occurrence of the aflatoxin M1 (AFM1) in raw milk. We analyzed 45 samples of raw bovine milk from expansion tanks from 23 farms in different municipalities of the dairy belt of the state of Alagoas/Brazil. Samples were collected directly from the cooling tanks and transported under refrigeration for analysis. The method used for the extraction of AFM1 was that proposed by the Adolfo Lutz Institute. On the other hand, the detection of AFM1 occurred by high performance liquid chromatography (HPLC) by identifying retention times. The results of the analyses indicated that none of the collected samples presented contamination by aflatoxin M1, thus indicating that the milk commercialized in Alagoas shows a good quality against this toxic agent.

Key words: Aflatoxin M1, bovine milk, Alagoas.