African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5092

Full Length Research Paper

Differential niche occupation and the biotechnological potential of Methylobacterium species associated with sugarcane plants

Pedro Avelino Maia de Andrade
  • Pedro Avelino Maia de Andrade
  • Department of Soil Science, ‘Luiz de Queiroz’ College of Agriculture, University of São Paulo, Piracicaba, SP, Brazil.
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Armando Cavalcante Franco Dias
  • Armando Cavalcante Franco Dias
  • Department of Soil Science, ‘Luiz de Queiroz’ College of Agriculture, University of São Paulo, Piracicaba, SP, Brazil.
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Simone Raposo Cotta
  • Simone Raposo Cotta
  • Department of Soil Science, ‘Luiz de Queiroz’ College of Agriculture, University of São Paulo, Piracicaba, SP, Brazil.
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Diogo Paes da Costa
  • Diogo Paes da Costa
  • Federal Rural University of Pernambuco, Garanhuns/Academic Unit of Garanhuns, Garanhuns, PE, Brazil.
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João Tiago Correia Oliveira
  • João Tiago Correia Oliveira
  • Federal Rural University of Pernambuco, Garanhuns/Academic Unit of Garanhuns, Garanhuns, PE, Brazil.
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Lucianne Ferreira Paes de Oliveira
  • Lucianne Ferreira Paes de Oliveira
  • Federal Rural University of Pernambuco, Garanhuns/Academic Unit of Garanhuns, Garanhuns, PE, Brazil.
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Fernando José Freire
  • Fernando José Freire
  • Department of Soil Science, Federal Rural University of Pernambuco, Recife, PE, Brazil.
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Fernando Dini Andreote
  • Fernando Dini Andreote
  • Department of Soil Science, ‘Luiz de Queiroz’ College of Agriculture, University of São Paulo, Piracicaba, SP, Brazil.
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Julia Kuklinsky-Sobral
  • Julia Kuklinsky-Sobral
  • Federal Rural University of Pernambuco, Garanhuns/Academic Unit of Garanhuns, Garanhuns, PE, Brazil.
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  •  Received: 27 May 2018
  •  Accepted: 19 June 2018
  •  Published: 07 July 2018

Abstract

This work highlighted a putative link between the physiological activity and genetic diversity of Methylobacterium species and the association with sugarcane roots and rhizoplane. In total, 40 isolates previously described as pink-pigmented facultative methylotrophic bacteria (PPFMs), were evaluated for their ability to fix nitrogen and solubilize inorganic phosphate, amylase and pectinase activity. This in vitro potential was positively correlated with the community isolated from the root tissues than those from the rhizoplane. Regarding the genomic fingerprinting, the (BOX-PCR) approach revealed a low similarity among the isolates, occurring sole 7 haplotypes harboring more than 70% of similarity among band patterns. These results revealed that the genomic fingerprinting of the isolates recovery from roots is different from the rhizoplane. Besides that, these haplotypes occurred on both sugarcane varieties. Using a phylogenetic sequencing approach based on the 16S rRNA gene, we observed a high abundance of sequences similar to Methylobacterium radiotolerans colonizing both plant tissue and sugarcane varieties were observed. Hence, it was suggested that the plant should select those Methylobacterium spp. with a high biotechnological potential to promote plant growth. Therefore, the bioprospection of specific endophytic bacterial groups comprise an important source of biotechnological potential to improve sugarcane growth and production.

Key words: Pink-pigmented facultative methylotrophic bacteria, plant growth promotion, BOX-PCR, 16S rDNA, Methylobacterium radiotolerans.