Full Length Research Paper
ABSTRACT
This work highlighted a putative link between the physiological activity and genetic diversity of Methylobacterium species and the association with sugarcane roots and rhizoplane. In total, 40 isolates previously described as pink-pigmented facultative methylotrophic bacteria (PPFMs), were evaluated for their ability to fix nitrogen and solubilize inorganic phosphate, amylase and pectinase activity. This in vitro potential was positively correlated with the community isolated from the root tissues than those from the rhizoplane. Regarding the genomic fingerprinting, the (BOX-PCR) approach revealed a low similarity among the isolates, occurring sole 7 haplotypes harboring more than 70% of similarity among band patterns. These results revealed that the genomic fingerprinting of the isolates recovery from roots is different from the rhizoplane. Besides that, these haplotypes occurred on both sugarcane varieties. Using a phylogenetic sequencing approach based on the 16S rRNA gene, we observed a high abundance of sequences similar to Methylobacterium radiotolerans colonizing both plant tissue and sugarcane varieties were observed. Hence, it was suggested that the plant should select those Methylobacterium spp. with a high biotechnological potential to promote plant growth. Therefore, the bioprospection of specific endophytic bacterial groups comprise an important source of biotechnological potential to improve sugarcane growth and production.
Key words: Pink-pigmented facultative methylotrophic bacteria, plant growth promotion, BOX-PCR, 16S rDNA, Methylobacterium radiotolerans.
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
CONCLUSION
The main goal of this study was to show that the association between sugarcane and Methylobacterium spp. are based on many specific traits; the in vitro potentials to promote plant development such as atmospheric nitrogen fixation, phosphate solubilization and extracellular enzyme activity, and specific genomic fingerprints, and not in relation to its taxonomical identity or phylogenetic distance. Hence, those are only some steps in the complete understanding of the interaction between Methylobacterium genus and sugarcane. The complete mechanisms that might determine this relationship are yet to be elucidated. For this reason, further genomic studies must be conducted in order to understand these interaction mechanisms between M. radiotolerans and sugarcane, and even more, their interaction under greenhouse conditions.
CONFLICT OF INTERESTS
The authors have not declared any conflict of interests.
ACKNOWLEDGEMENTS
The authors thank CNPQ (180203/2008-8) and (142344/2013-3) for awarding the research grant to Pedro Avelino Maia de Andrade as well as the Rural Federal University of Pernambuco and the Soil Sciences Department at the College of Agriculture “Luiz de Queiroz” University of São Paulo (Esalq-USP). They also acknowledge the critical review of Msc. Priscila Alves Giovani.
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