African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Plant growth promoting capability of Azotobacter as mono and mix culture on Vigna radiata

Munnaza Kiran, Shazia Afrasayab, Zaigham Abbas*, Muhammad Faisal and Shahida Hasnain
Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan.
Email: [email protected]

  •  Accepted: 17 January 2012
  •  Published: 16 February 2012

Abstract

In the present study mono and mixed culture bacterial combinations of Azotobacter were used to inculcate Vigna radiata seeds. In general, bacterial inoculations (mono and mixed cultures) promoted seed germination, early growth parameters, auxin content, soluble protein content, peroxidase and acid phosphatase activity relative to non-inoculated control seedlings. Increase of (23%) as well as decrease (6.8%) in the root length were observed with bacterial inoculations relative to non-inoculated seedlings. About 20.07% enhancements in fresh weight and 62% enhancement in dry weight was observed in case of bacterial inoculations. Among the monoculture Ab-4(A3) induced pronounced growth stimulator effects, mixed culture combinations B6, B7, and B10 showed pronounced synergistic effects relative to respective monocultures, while B2 exhibited negative in majority of the parameters being studied.

 

Key words: Azotobacter, Vigna radiata, auxin, acid phosphatase, peroxidase.

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