African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4875

Full Length Research Paper

GC-MS and molecular analyses of monocrotophos biodegradation by selected bacterial isolates

Buvaneswari G.
  • Buvaneswari G.
  • PG and Research Department of Microbiology, JJ College of Arts and Science (Autonomous), Sivapuram, Pudukkottai, Tamil Nadu, India.
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Thenmozhi R.
  • Thenmozhi R.
  • PG and Research Department of Microbiology, JJ College of Arts and Science (Autonomous), Sivapuram, Pudukkottai, Tamil Nadu, India.
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Nagasathya A.
  • Nagasathya A.
  • Goverment Arts College for Women, Pudukkottai, Tamil Nadu, India.
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Thajuddin N.
  • Thajuddin N.
  • Department of Microbiology, Bharathidasan University, Tiruchirapalli, Tamil Nadu, India.
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Praveen Kumar D.
  • Praveen Kumar D.
  • Contec Global Agro Limited (Contec Global Group), Abuja, Nigeria.
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  •  Received: 31 August 2017
  •  Accepted: 30 November 2017
  •  Published: 21 January 2018

Abstract

Soil treatment incubation method was carried out to analyze the monocrotophos (MCP) biodegradation using three selected bacterial isolates namely Pseudomonas stutzeri (KY287931), Bacillus licheniforms (KY287928), and Bacillus sonorensis (KY287930). After 45 days of incubation of pesticide exposed to soil samples, the MCP biodegradation by individual cultures and consortium culture was analyzed and conformed by gas chromatography–mass spectrometry (GC-MS) method. The three isolates individually degrade toxic MCP into non-toxic intermediate while the consortium cultures completely degrade the MCP into water-soluble compounds. PCR-based RFLP analysis of 16S rRNA was performed. Pseudomonas and Bacillus species have been phylogenetically analyzed by using 16S rRNA gene sequences. The isolated bacterial consortium culture proved a potential agent for MCP biodegradation.

Key words: Soil, MCP-monocrotophos, bacterial isolate, gas chromatography–mass spectrometry (GC-MS).