Soil treatment incubation method was carried out to analyze the monocrotophos (MCP) biodegradation using three selected bacterial isolates namely Pseudomonas stutzeri (KY287931), Bacillus licheniforms (KY287928), and Bacillus sonorensis (KY287930). After 45 days of incubation of pesticide exposed to soil samples, the MCP biodegradation by individual cultures and consortium culture was analyzed and conformed by gas chromatography–mass spectrometry (GC-MS) method. The three isolates individually degrade toxic MCP into non-toxic intermediate while the consortium cultures completely degrade the MCP into water-soluble compounds. PCR-based RFLP analysis of 16S rRNA was performed. Pseudomonas and Bacillus species have been phylogenetically analyzed by using 16S rRNA gene sequences. The isolated bacterial consortium culture proved a potential agent for MCP biodegradation.
Key words: Soil, MCP-monocrotophos, bacterial isolate, gas chromatography–mass spectrometry (GC-MS).
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