African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4958

Full Length Research Paper

Molecular detection and prevalence of Toxoplasma gondii in pregnant women in Sudan

Maha H. Elamin1, Ebtesam M. Al-Olayan1, Sawsan A. Omer1, Abdulaziz N. Alagaili2and Osama B. Mohammed2*      
1Department of Zoology, College of Science, King Saud University, University Centre for Women Students, P. O. Box 22452, Riyadh 11495, Saudi Arabia. 2KSU Mammals Research Chair, Department of Zoology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
Email: [email protected]

  •  Accepted: 14 December 2011
  •  Published: 16 January 2012

Abstract

The aim of the present study was to determine the prevalence of Toxoplasma gondiiantibodies in serum samples from two groups of women in Sudan and to use molecular techniques for detection of the organism’s DNA using B1 gene. The first group (study group) composed of 94 individuals and they aborted during the study, while the second group (control group) also composed of 94 individuals who gave birth to healthy children without complications. Serological screening using enzyme linked immunosorbent assay (ELISA) kits resulted in 39.4% and 35.1% of the women in the control and study group showed positive IgG antibodies to T. gondii respectively. PCR amplification of B1 gene resulted in detection of PCR product in 19.1% of the study group and 22.3% of the control group. There was no association between positive toxoplasmosis antibodies and abortion, furthermore, the individuals with positive PCR results in the control group did not abort. The usefulness of the B1 gene as a target for toxoplasmosis diagnosis was documented and the possible source of infection in the control and the study groups was discussed.

Key words: Toxoplasma gondii, antibodies, women, Sudan, B1 gene, abortion.

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