African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4910

Full Length Research Paper

Biochemical and molecular identification of newly isolated pigmented bacterium and improved production of biosurfactant

Helvia W. Casullo Araujo
  • Helvia W. Casullo Araujo
  • Department of Chemistry, State University of Paraíba, 58429-500, Campina Grande, Paraíba, Brazil.
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Rosileide F. S. Andrade
  • Rosileide F. S. Andrade
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife, Pernambuco, Brazil.
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Dayana Montero-Rodríguez
  • Dayana Montero-Rodríguez
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife, Pernambuco, Brazil.
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Vanessa Pimentel Santos
  • Vanessa Pimentel Santos
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife, Pernambuco, Brazil.
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Patricia C. V. Souza Maia
  • Patricia C. V. Souza Maia
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife, Pernambuco, Brazil.
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Carlos F. B. Costa Filho
  • Carlos F. B. Costa Filho
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife, Pernambuco, Brazil.
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Carlos A. Alves da Silva
  • Carlos A. Alves da Silva
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife, Pernambuco, Brazil.
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Galba Maria Campos-Takaki
  • Galba Maria Campos-Takaki
  • Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590, Recife, Pernambuco, Brazil.
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  •  Received: 18 October 2016
  •  Accepted: 17 November 2016
  •  Published: 14 June 2017

Abstract

A novel bacterium pigmented isolate from Caatinga soil was characterized by biochemical and molecular assays, as well as, by rep-polymerase chain reaction (PCR) and 16S rDNA sequencing, and was identified as Serratia marcescens based on 99% of similarity. The identity of the sequences were compared by pairs of critical species of S. marcescens found in National Center for Biotechnology Information (NCBI) with a 96% homology of the isolated species to species database. The wild strain was able to produce biosurfactant (BS) using cassava (Manihot esculenta Crantz) wastewater, with addition of lactose, and corn oil, according to full factorial design 23, at 28°C, and static condition. The net liquid metabolic reduced the surface tension of the water from 70 to 30.60 mN/m during the stationary phase (assay eight constituted by 6.0% cassava wastewater, 1.0% lactose and 7.5% corn oil), and produced emulsifier agent (4.012 UEA) at the same condition. This study identified the pigmented bacterium as new strain of S. marcescens, and showed it has potential to promote both emulsions formation and surface tension reduction in cassava wastewater (6.0%), lactose (1.0%) and corn oil (7.5%) proved as an alternative economical medium for commercial biosurfactant processes.

 

Key words: Serratia marcescens, tensio-active, bioemulsifier, cassava wastewater.