African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5227

Full Length Research Paper

Study on microbial community and diversity of an abscisic acid wastewater anaerobic granular sludge system

Xijin Zhao1,2,  Ping Wang1*, Yunyi Yao3, Qinghua Tian2, Wei Wang2, Lei Jian2 and Binzhong Yang2
1College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China. 2Sichuan Academy of Environmental Sciences, Chengdu 610041, China. 3Research Center for Biochemistry and Molecular Biology, Jiangsu Key Laboratory of Brain Disease Bio-information, Xuzhou Medical College, Xuzhou 221004, P. R. China.
Email: [email protected]

  •  Accepted: 24 September 2012
  •  Published: 25 December 2012


The microbial community and diversity in an abscisic acid wastewater anaerobic granular sludge system were characterized using molecular techniques. 16S rRNA clone library and sequence analysis revealed that about 99% of 161 bacterial clones belonged to theFirmicutes and Proteobacteria, the remaining 2 clones were assigned to Bacteroidetes.Clostridia (34.8%) and Bacilli (32.3%) belonged to Firmicutes; in the class ofProteobacteria, the clones were assigned to Betaproteobacteria (15.5%),Gammaproteobacteria (13.7%), Alphaproteobacteria (1.2%) and Epsilonproteobacteria(1.2%).  For the domain of Archaea, 37.35% of 83 archaeal clones were affiliated withMethanospirillum and Methanocorpusculum respectively; the other of 25.3% belonged toMethanosphaerula.


Key words: Abscisic acid wastewater, microbial community and diversity, 16s rRNA, up-flow anaerobic sludge blanket.