Abstract

The microbial community and diversity in an abscisic acid wastewater anaerobic granular sludge system were characterized using molecular techniques. 16S rRNA clone library and sequence analysis revealed that about 99% of 161 bacterial clones belonged to theFirmicutes and Proteobacteria, the remaining 2 clones were assigned to Bacteroidetes.Clostridia (34.8%) and Bacilli (32.3%) belonged to Firmicutes; in the class ofProteobacteria, the clones were assigned to Betaproteobacteria (15.5%),Gammaproteobacteria (13.7%), Alphaproteobacteria (1.2%) and Epsilonproteobacteria(1.2%).  For the domain of Archaea, 37.35% of 83 archaeal clones were affiliated withMethanospirillum and Methanocorpusculum respectively; the other of 25.3% belonged toMethanosphaerula.

Key words: Abscisic acid wastewater, microbial community and diversity, 16s rRNA, up-flow anaerobic sludge blanket.