December 2012
Efficacy of probiotics in improving water quality and bacterial flora in fish ponds
Fish culture aimed at high production through intense culture practices often leads not only to the alteration in water quality but also to severe disease problems. The accumulation of organic wastes deteriorates water quality and encourages the proliferation of pathogenic microorganisms. To avoid these problems, probiotics are used as bioremediation tool. The present study was aimed to know the changing...
December 2012
Isolation and molecular identification of polyaromatic hydrocarbons- utilizing bacteria from crude petroleum oil samples
Polyaromatic hydrocarbons (PAHs) are worldwide contaminants that can reach toxic concentrations that are detrimental to the environment and human health. In this study, three different isolates (Klebsiella oxytoca, Klebsiella pneumonia and Acinetobacter sp.) from crude petroleum oil samples were found to be PAHs utilizers. They are able to utilize four different PAHs, phenanthrene, fluoranthene,...
December 2012
Antimicrobial potential of licorice: Leaves versus roots
Medicinal plants play a vital role in covering the basic health needs. They may offer a new source of antibacterial agents. The aim of this study was to screen in-vitro theantimicrobial activity of some Egyptian medicinal plants against clinical methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from different hospitals in Egypt followed by studying the MIC and cytotoxicty...
December 2012
Selection of probiotic strains and development of green tea based probiotics for livestock
Twenty six (26) strains of Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus casei, Enterococcus faecium, Bacillus subtilis, Bacillus coagulans, andSaccharomyces cerevisiae from Korean Collection for Type Cultures (KCTC) were evaluated in this experiment for the development of green tea based probiotics production and their inhibition effect against pathogenic bacteria was examined....
December 2012
Frequency and distribution of genome-based microsatellites in Verticillium dahliae
A total of 5,418 simple sequence repeats (SSRs) were identified in the 33.8 Mb genomic DNA sequence of Verticillium dahliae. SSR loci were classified by repeat types and frequency in different genomic regions. The results show that the SSRs in different repeat units exhibited differential or non-random distribution in different genomic locations. Whole genome analyses showed that the tri-nucleotide (nt) repeat...
December 2012
Specific DNA markers for detection of bacterial canker of kiwifruit in Sichuan, China
Bacterial canker of kiwifruit (Actinidia chinensis) has become one of the most serious factors that limit the cultivation of kiwifruit in Sichuan, China. Therefore, it is urgent to develop a rapid and precise method to detect the disease at its early infection stage. A 1300 bp specific fragment of pathogenic bacteria was obtained by random amplified polymorphic DNA (RAPD) analysis. This fragment was cloned and...
December 2012
Immunogenicity of a tuberculosis DNA vaccine expressing a fusion protein Ag85B - MPB64 in mice
Tuberculosis remains a major infectious disease worldwide. There are no effective vaccines developed for the disease so far and the efficacy of the only available vaccine, Mycobacterium bovis Bacillus Calmette–Guérin (BCG) is generally low against Mycobacterium tuberculois(MTb). DNA vaccine is especially becoming promising and novel vaccine approach. In this study, a plasmid DNA vaccine,...
December 2012
Antimicrobial resistance of Salmonella spp. strains isolated from hamburgers
Susceptibility of 45 Salmonella spp. strains isolated from beef and beef and chicken hamburgers was evaluated. Twelve antimicrobial drugs were used in the disc diffusion method. A cladogram was constructed and similarity across strains was assessed. Strains were susceptible to co-trimoxazole, chloramphenicol, amikacin, tetracycline and gentamicin (91.11, 91.11, 84.44, 80.0, 77.78%), though resistance was...
December 2012
In vitro screening for oil degrading bacteria and evaluation of their biodegradation potential for hydrocarbon
Twenty seven potential hydrocarbon degrader bacterial isolates were isolated from soil that has been exposed to crude petroleum oil. The hydrocarbon degradation potentialities of these isolates were assessed against n-tetradecane. Four isolates (AT3, AT5, AT11 and AT15) were the most potent isolates. These isolates were identified via morphological, biochemical and 16S-rRNA gene sequencing techniques as...
December 2012
Cloning and expression analysis of accD gene in Chlorella protothecoides FACHB-2
Acetyl-CoA carboxylase (ACCase) is one of the key enzymes that participate in lipid biosynthesis; a detailed understanding of its regulation mechanism is of potential value for lipid production in oil-producing species. In this study, a 1.5 kb fragment of accD gene from Chlorella protothecoides FACHB-2 was cloned, and the expression levels of it under autotrophic and heterotrophic conditions...
December 2012
Study on microbial community and diversity of an abscisic acid wastewater anaerobic granular sludge system
The microbial community and diversity in an abscisic acid wastewater anaerobic granular sludge system were characterized using molecular techniques. 16S rRNA clone library and sequence analysis revealed that about 99% of 161 bacterial clones belonged to theFirmicutes and Proteobacteria, the remaining 2 clones were assigned to Bacteroidetes.Clostridia (34.8%) and Bacilli (32.3%) belonged...
December 2012
Diversity of epiphytic fungi on the diseased and healthy leaves of Bambusa
Based on the sampling on permanent sample plots in different seasons, quantities of epiphytic fungi, seasonal changes of fungal species, fungal community composition, and the relationship of epiphytic fungi and microenvironment of Bambusa pervariabilis ×Dendrocalamopisis grandis were studied by plate dilution, morphological and molecular biological methods. The above four aspects were also...
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