African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5092

Full Length Research Paper

Extended spectrum beta-lactamase production and plasmid mediated quinolone resistance among lactose non-fermenting nterobacteriaceae isolated from poultry sources in Calabar, Nigeria

Nchawa Yangkam Yhiler
  • Nchawa Yangkam Yhiler
  • Department of Medical Laboratory Science, Faculty of Allied Medical Science, University of Calabar, Calabar, Nigeria.
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Bassey Enya Bassey
  • Bassey Enya Bassey
  • Department of Medical Laboratory Science, Faculty of Allied Medical Science, University of Calabar, Calabar, Nigeria.
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Inyang-etoh Paul
  • Inyang-etoh Paul
  • Department of Medical Laboratory Science, Faculty of Allied Medical Science, University of Calabar, Calabar, Nigeria.
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Useh Monday Francis
  • Useh Monday Francis
  • Department of Medical Laboratory Science, Faculty of Allied Medical Science, University of Calabar, Calabar, Nigeria.
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Asuquo Anne
  • Asuquo Anne
  • Department of Medical Laboratory Science, Faculty of Allied Medical Science, University of Calabar, Calabar, Nigeria.
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Angela Okocha-Ejeko
  • Angela Okocha-Ejeko
  • World Health Organization, UN house, Plot 617/618, Central Area District, FCT, Abuja, Nigeria.
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  •  Received: 08 May 2019
  •  Accepted: 02 July 2019
  •  Published: 31 July 2019

Abstract

This study investigated the co-carriage of plasmid mediated quinolone resistance (PMQR) and extended spectrum beta-lactamase (ESBL) producing lactose non-fermenting (LNF) Enterobacteriaceae isolated from poultry birds. This was a descriptive cross-sectional study carried out between September, 2016 and March, 2017. The Kirby-Bauer disk diffusion method was used to determine the antimicrobial susceptibility patterns. ESBL screening disc kit was used to detect ESBL activities. Detection of ESBL and PMQR genes was carried out by means of polymerase chain reaction. In total, 207 LNF Enterobacteriaeae isolates were recovered from the cloacal swabs of poultry birds within the Calabar Metropolis. ESBL-producing isolates were 162 (78.3%) while fluroquinolone resistant isolates were 194 (93.7%). Among the ESBL-producing isolates, resistance to Ciprofloxacin, Norfloxacin, Levofloxacin, Ofloxacin and Nalidixic acid was 55 (34.2%), 26 (16.1%), 35 (21.7%), 50 (31.1%), and 162 (100%), respectively. About 65% of the quinolone resistant isolates were positive for at least one of the PMQR and ESBL genes in this study. Strict antimicrobial screening, surveillance of resistant isolates as well as the judicious practice of antimicrobial administration in the poultry setting with special emphasis on fluoroquinolones is advised given the high prevalence of co-existent ESBL and PMQR genes.

 

Key words: LNF enterobacteriaeae, Extended spectrum beta-lactamases, quinolone resistance