African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5223

Full Length Research Paper

Molecular identification and assessment of genetic diversity of fluorescent pseudomonads based on different polymerase chain reaction (PCR) methods

Inès Mehri1*, Yousra Turki1, Imen Daly1, Asma Ben Rjab1, Abdennaceur Hassen1and Maher Gtari2
1Laboratoire Traitement et Recyclage des Eaux, Centre de Recherche et des Technologies des Eaux, Borj-Cédria, Tunisia. 2Laboratoire Microorganismes et Biomolécules actives Faculté des Sciences de Tunis, Campus Universitaire, 2092, Tunis, Tunisia.
Email: [email protected]

  •  Accepted: 15 February 2013
  •  Published: 07 May 2013

Abstract

A total of 66 fluorescent pseudomonads strains isolated from diverse Tunisian environmental biotypes (wastewater, compost, wastewater treatment plant, etc.) were analysed by two polymerase chain reaction (PCR)-based methods, 16S-23S intergenic spacer regions (ITS)-PCR and repetitive extragenic palindromic (BOX)-PCR. These typing techniques were evaluated to assess their usefulness as tool to study thePseudomonas diversity within this complex group. Genetic analysis using ITS- and BOX-PCR generated respectively 12 and 45 distinct profiles. Phylogenetic relationships within fluorescent pseudomonads were examined by analyzing partial 16S rRNA and rpoBgenes sequences. The phylogenetic resolution of the rpoB tree was higher than that of the 16S rRNA tree. Moreover, the sequencing of the rpoB gene has recognized 13 different species and sub-species, while the 16 rRNA gene sequencing differentiated only 9 species.

 

Key words: Fluorescent pseudomonads, 16S-23S intergenic spacer regions (ITS)-PCR, repetitive extragenic palindromic (BOX)-PCR, 16S rRNA, rpoB.