May 2013
Comparison of chromogenic agar medium and diffusion disk test for detection of hospital acquired methicillin resistant Staphylococcus aureus (HA-MRSA) from patients and hospital environment in Nasiriyah city, Iraq
To compare the culture screening protocols for detection of methicillin-resistantStaphylococcus aureus (MRSA) in the surgical wards, one hundred and forty eight samples (116 nasal swabs from 116 patients and 32 environment items swabs) were examined. 96 (64.8%) of the samples stowed S. aureus, 75 (64.6%) from patients, and 21(65.6%) from hospital environment. The detection of MRSA among the isolates of the S....
May 2013
Multidrug resistant microorganisms causing neonatal septicemia: In a tertiary care hospital Lahore, Pakistan
Septicemia is an important cause of morbidity and mortality in neonates. Appropriate clinical diagnosis and empirical treatment is crucial as pathogens causing sepsis and their antibiotic susceptibility pattern varies in different settings. Therefore, this study was designed to determine the pattern of organisms causing neonatal sepsis and their antimicrobial resistance profile. One hundred and three blood culture...
May 2013
Prevalence of Listeria species and Listeria monocytogenes serotypes in ready mayonnaise salads and salad vegetables in Iran
Three hundred samples of ready mayonnaise salads and salad vegetables were used forisolation of Listeria spp. by ISO 11290-1. The isolates were identified using biochemical tests, further confirmed by duplex PCR and differentiated via conventional agglutination serotyping. A total of 8.7% of the samples harboured Listeria spp., including 7% Listeriamonocytogenes, 1% Listeria...
May 2013
Assessment of the antistaphylococcal activity of ethanolic extract of propolis (EEP)
The aim of this study was to determine in vitro conditions the antimicrobial activity of Ethanolic Extract of Propolis (EEP), originating from Poland, in relation to 23 variousStaphylococcus spp. strains depending on time of its activity. The conducted research shows that examined EEP demonstrated the highest activity in the twentieth hour of research although, all the analyzed strains displayed MIC value...
May 2013
Development of PCR method for fast detection of Ophiostoma floccosum in wood chips
Ophiostoma floccosum is one of blue stain fungi that cause cosmetic damage in softwood. To develop a molecular maker for the detection of O. floccosum, we designed Oflo1 and Oflo2 primers based on the β-tubulin gene. PCR assay with eleven isolates ofO. floccosum and nine other blue stain and discoloring fungal species (moulds) proved that the designed primers specifically amplified a 333 bp...
May 2013
In vitro study of the antifungal efficacy of zinc oxide nanoparticles against Fusarium oxysporum and Penicilium expansum
The antifungal efficiency of Zinc oxide nanoparticles (ZnO NPs) was investigated against two pathogenic fungal species, F. oxysporum and P. expansum. The two fungi were identified at molecular level by nuclear ribosomal DNA internal transcribed spacer (ITS) identities. They have been submitted to the GenBank with accession numbers of AB753032 and AB753033 for F. oxysporum and P....
May 2013
Growth responses of hydroponic green fodder system and Fusarium oxysporum to external supply of mycolytic enzymes applied on seaweed bagasse as carrier
High humidity, nutritional and environmental conditions involved in hydroponic green fodder (HGF) production increases fungal contamination risk. Chemical fungicides application is limited because of their toxicity to animals. An alternative biotechnological option is the use of mycolytic enzymes such as chitinase and laminarinase. The goal of the present study was to immobilize chitinase and laminarinase on seaweed...
May 2013
Medium optimization for production of antifungal active substances from antagonistic Brevibacillus laterosporus and its antifungal activity analysis
The antagonist bacterium Brevibacillus laterosporus ZQ2 with broad-spectrum antifungal activity was isolated from an apple rhizosphere at Mount Tai in China. The fermentation medium for the production of antifungal substances from ZQ2 was statistically optimized. In one-factor-at-a-time experiments, five components, including carbon sources, nitrogen sources, and inorganic salt, were selected. Two factors were...
May 2013
Seasonal variation of soil environmental characteristics affect the presence of Burkholderia pseudomallei in Khon Kaen, Thailand
The environmental Gram negative bacteria Burkholderia pseudomallei, the causative agent of melioidosis, have been found globally. B. pseudomallei can survive under a wide range of environmental conditions and is isolated from soil in both rainy and dry seasons. The correlation of melioidosis cases have been predominantly found present in B. pseudomallei during rainfall. In the present study,...
May 2013
Mixed sexually transmitted infections in adult male patients with urethral discharge and female patients with pelvic inflammatory disease
To determine the percentage of mixed sexually transmitted infections in a high risk sample population of men and women, urethral and endocervical swabs were collected from 200 men with urethritis and 200 women with Pelvic Inflammatory Disease (PID). Cell cultures, plate culture and microscopy were used to detect the presence of Neisseria gonorrhoeae, Chlamydia trachomatis and Herpes simplex virus (HSV). Serological...
May 2013
Growth responses of Triticum aestivum after inoculating with Pseudomonas and Stenotrophomonas
Three bacterial strains (Pseudomonas species, P1; Pseudomonas mendocina, P2; andStenotrophomonas maltophilia, S3) were investigated in this study to assess their growth stimulatory effects on Triticum aestivum plants. These strains are Gram negative, motile, aerobic rods with catalase and oxidase positive. In general, seed inoculations of these bacteria (mono and mixed cultures) promoted germination,...
May 2013
Assessment of microbiological indoor air quality in public buildings: A case study (Timisoara, Romania)
The indoor air quality in public buildings is essential for the health of employees and visitors. To investigate the potential influence of airborne germ loads on human health, two sampling campaigns were conducted during 2009 in several public buildings in Timisoara (Romania). The quality of air revealed highly significant differences among different sites. Cluster analysis accurately classified the investigated...
May 2013
Induction of yeast cells from sporangiospores of Rhizopus stolonifer was inhibited by sodium fluoride
This study was aimed at determining the effect of the glycolytic inhibitor, sodium fluoride(NaF), on induced yeast cells. Rhizopus stolonifer was cultivated in synthetic broth for 120 h, at pH 4.5 and ambient temperature of 28 ± 1°C, using sporangiospores as inoculum.Growth was in discrete units, which were sedimented. Microscopic examination revealed induction of neoplasts,...
May 2013
Antimicrobial potentiality of Polyalthia longifolia seed oil against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin
The antimicrobial potentiality of Polyalthia longifolia seed oil and its fatty acid methyl ester (FAME) extract was evaluated against multi drug resistant strains of bacteria and fungi of clinical origin. A sensitivity test was performed to determine the resistance pattern for marketed antimicrobial agents against the selected microorganisms. The results of antimicrobial assay revealed that P....
May 2013
Identification of fibrinogen-induced nattokinase WRL101 from Bacillus subtilis WRL101 isolated from Doenjang
To increase the fibrinolytic enzyme (nattokinase WRL101) in Bacillus subtilis WRL101, bovine fibrin or fibrinogen (1.0%, w/v) were added in the tryptic soy broth as a substrate. The fibrinolytic activity cultured in the fibrinogen-contained medium was increased. On the other hand, fibrin decreased the activity. Using the medium condition, nattokinase WRL101 (fibrinogen-induced nattokinase WRL101, FIN-WRL101)...
May 2013
Evaluation of microbial elicitors to induce plant immunity for tomato wilt
The bioactive compounds of Chaetoglobosin C, chaetomanone A and trichotoxin mixture used as microbial elicitors to elicit a-tomatine in tomato was investigated. The a-tomatinewas detected from tomato seedlings var. Sida sprayed with chaetoglobosin C, chaetomanone A and trichotoxin mixture, followed by the inoculation with Fusarium oxysporum f sp lycopersici. Results show that disease...
May 2013
Isolation and identification of an effective fibrinolytic strain Bacillus subtilis FR-33 from the Chinese doufuru and primary analysis of its fibrinolytic enzyme
Fibrinolytic microorganisms were screened from the Chinese soy cheese doufuru as well as Japanese natto and Chinese douchi. Initially, 16 isolates with fibrinolytic activity were selected based on activity and cultural characteristics for further characterization. Anexponential standard curve, covering 39 to 2500 IU/mL of urokinase activity, was well established...
May 2013
Seroprevalence of Chlamydia infection in dairy cattle in subtropical southern China
A total of 875 serum samples from dairy cattle in six districts in Zhuang Autonomous Region (GZAR) were collected between June 2009 to March 2010, and Chlamydiaantibodies were assayed by indirect hemagglutination test (IHA). The overall seroprevalence of Chlamydia in dairy cattle was 17.83% (156/875) in GZAR. The seroprevalence of Chlamydia in aborting dairy cattle (18.18%) was higher than that...
May 2013
Isolation and identification of oleaginous endophytic fungi
Endophytic fungi are a rich source of novel organic compounds with interesting biological activities and a high level of biodiversity. A total of 76 endophytic fungus strains were isolated from 13 species of plants. Through a preliminary screening and fermentation assay, a fungi named ML-GEN.1 isolated from Strobilanthes cusia was found and the lipid content reached 59%. This is the first report of oleaginous...
May 2013
Bacterial contamination in kitchens of rural and urban areas in Meerut district of Utter Pradesh (India)
A total of 80 samples were collected from kitchens of rural and urban areas in Meerut district during July to August 2010. The more bacterial contamination (97.5%) in kitchens of rural areas as compared to the kitchen of urban areas (80%) was reported. The total number of bacteria isolates from the air of kitchens in rural and urban areas was 69 and 52, respectively, with total number of bacterial strain (66). The...
May 2013
Potentials of walnut (Tetracarpidium conophorum Mull. Arg) leaf and onion (Allium cepa Linn) bulb extracts as antimicrobial agents for fish
The development of antibiotic-resistant pathogens due to the indiscriminate use of antibiotics has led to advocacy for the use of natural products in the treatment of fishdiseases. The antimicrobial activity of methanolic and ethanolic extracts of walnut leaves and onion bulbs were evaluated against six pathogenic bacteria (Pseudomonas...
May 2013
Isolation and characterization of gut-associated microbes in cockroach
Arthropods, particularly insects form successful highly symbiotic relationship with endosymbiotic bacteria. The associations between insects and endosymbionts are remarkably stable; many stretch back several hundred million years in evolutionary time.Insects are amongst the most successful of animals, both in terms of diversity and in colonizing all ecological niches. The aim of this study was to isolate and identify...
May 2013
The frequency and antimicrobial sensitivity pattern of extended spectrum β-lactamase (ESBLs) producing gram negative bacilli isolated from urine in a tertiary care hospital of Pakistan
Urinary tract infections (UTIs) are one of the most common bacterial infections in humans, both in the community and the hospital settings. An extensive use of β -lactam antibiotics in hospitals and community has created major resistance problem leading to increased morbidity, mortality and health-care costs. Local knowledge of antimicrobial susceptibilities of these organisms is important for implementation of...
May 2013
A case of malignant melanoma patient complicating pneumonia of Legionella pneumophila Sg 1 infection and means to detect Legionella from the patient
Legionella pneumonia is mainly caused by the inhalation of Legionella contaminated aerosol, aspiration or wound infection. Patients undergoing a severe immunosuppressive therapy such as organ transplant or cancer therapy are particularly at risk of infection.This study reported a case of Legionella pneumonia caused by Legionella pneumophila in a man with malignant melanoma. The...
May 2013
Influence of incubation temperature, ph and food substrate on production of Bacillus cereus extracellular proteins and toxins
A total of 422 isolates of Bacillus cereus, isolated from fresh vegetables were characterized alongside a reference strain (NRRL-B14726) and conditions influencing thier extracellular protein and toxin production were evaluated. Optimal temperature and pH conditions for growth of the isolates were 28 to 37°C and pH 6 to 7, respectively. Under these...
May 2013
Prevalence of Listeria species in raw milk in Esfahan Province, Iran
Listeria sp. is one of the most important zoonotic diseases which cause dangerous illness. It consists of six species but the most important one is Listeria monocytogenes,which is a major concern for the food industry. Listeria is ubiquitous in dairy farms and it is isolated from cows’ milk. The purpose of this study is to identify Listeria spp. in 986 raw whole cow milk samples...
May 2013
Insights into the characterization of ars genes in the biomining bacterium Acidithiobacillus caldus SM-1
Acidithiobacillus caldus is an extremely acidophilic, moderately thermophilic, chemoautotrophic bacterium, which has been used to treat gold-bearing arsenopyrite ores. The arsenic resistance system (ars operon) was responsible for arsenic resistance of A. caldus. To investigate the characterization of ars genes, we analyzed 12 ars genes in A. caldus SM-1 using the...
May 2013
Production of glutamic acid by Corynebacterium glutamicum using dates syrup as carbon source
The present study was an illustrative investigation on L-glutamic acid production by usingCorynebacterium glutamicum employing a cheaply available dates syrup. Owing to the high sugar content of dates syrup (total sugar 80%) attempt was made to utilize the date syrup for the production of glutamic acid, in shaking culture. The acid treated dates syrup (ATDS) at concentration of 100g/L was the best sugar...
May 2013
Ultrastructural studies on the interaction between Bacillus subtilis MBI 600 (Integral®) and the rice sheath blight pathogen, Rhizoctonia solani
The present study evaluated interactions of the biological control agent Bacillus subtilisstrain MBI 600 (Integral®) and Rhizoctonia solani, the rice sheath blight pathogen, through scanning electron microscopy (SEM). Ultrastructural effects related to antibiosis were studied by dual culture of B. subtilis (MBI 600) and R. solani on potato dextrose agar (PDA) plates....
May 2013
Screening of plant growth promoting rhizobacteria for the development of vegetable crops inoculants
The enrichment of plant rhizosphere with beneficial bacteria is a strategy that favors the production of more vigorous seedlings, which is essential for the success of vegetable cultivation. The aim of this study was to screen fluorescent pseudomonads that are able to act as plant growth-promoting rhizobacteria. Production of indole acetic acid (IAA) and phosphate solubilization were used as traits for initial screening...
May 2013
Physiological behavior and antibiotic response of soybean (Glycine max L.) nodulating rhizobia isolated from Indian soils
A total of 92 soybean nodulating rhizobia were isolated from different agro regions and screened for sensitivity against 8 rhizobiophages. Only 33 showed susceptibility with phage strains. Nineteen rhizobial isolates selected, including 9 isolates having high lytic behaviour and 10 resistant to all phages, were subjected to study on symbiotic effectiveness. Strains ALSR12, KNSR16, USDA123 and USDA94...
May 2013
Molecular identification and assessment of genetic diversity of fluorescent pseudomonads based on different polymerase chain reaction (PCR) methods
A total of 66 fluorescent pseudomonads strains isolated from diverse Tunisian environmental biotypes (wastewater, compost, wastewater treatment plant, etc.) were analysed by two polymerase chain reaction (PCR)-based methods, 16S-23S intergenic spacer regions (ITS)-PCR and repetitive extragenic palindromic (BOX)-PCR. These typing techniques were evaluated to assess their usefulness as tool to study...
May 2013
Enzymatic activity, surface hydrophobicity and biogenic amines production in lactic acid bacteria isolated from an artisanal Spanish cheese
The enzymatic activities of 24 lactic acid bacteria strains have been studied. Lactobacilli strains exhibited the highest β-galactosidase and aminopeptidase activities. Acid phosphatase activity was high in lactobacilli and lactococci strains. Esterase activity was observed for leuconostoc and lactobacilli strains. The hydrophobicity test and the biogenic amines detection were also tested in some strains selected...
May 2013
Using a real-time quantitative polymerase chain reaction (PCR) method for reliable enumeration of Aeromonas hydrophila in water samples
Aeromonas hydrophila is an opportunistic pathogen of human and animals with a multifactorial pathogenesis. Control of A. hydrophila numbers in water is recommended for water quality estimation. Consequently, we developed a SYBR Green method for rapid quantification of A. hydrophila, targeting three genes: 16SrRNA gene, the cytolytic enterotoxin gene (aerA) and the heat-stable cytotonic...
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