African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5056

Full Length Research Paper

First novel phages from rodents with lytic activity on clinical Enterobacteriaceae strains: Initiation for phage therapy in West Africa

Kakou-Ngazoa E. Solange
  • Kakou-Ngazoa E. Solange
  • Plateforme de Biologie Moléculaire, Pasteur Institute, Abidjan, Côte d’Ivoire, BP 490 Abidjan, Cote d’Ivoire.
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Addablah A. Audrey
  • Addablah A. Audrey
  • Plateforme de Biologie Moléculaire, Pasteur Institute, Abidjan, Côte d’Ivoire, BP 490 Abidjan, Cote d’Ivoire.
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Krylova Katerina
  • Krylova Katerina
  • Laboratoire de Microbiologie, Institut Armand Frappier, Laval, 531 Boul. des Prairies Laval (Québec), H7V 1B7, Canada.
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Saraka Daniel
  • Saraka Daniel
  • Plateforme de Biologie Moléculaire, Pasteur Institute, Abidjan, Côte d’Ivoire, BP 490 Abidjan, Cote d’Ivoire.
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Kouassi K. Stéphane
  • Kouassi K. Stéphane
  • Plateforme de Biologie Moléculaire, Pasteur Institute, Abidjan, Côte d’Ivoire, BP 490 Abidjan, Cote d’Ivoire.
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Coulibaly Ngolo David
  • Coulibaly Ngolo David
  • Plateforme de Biologie Moléculaire, Pasteur Institute, Abidjan, Côte d’Ivoire, BP 490 Abidjan, Cote d’Ivoire.
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Sina Kouamé Mireille
  • Sina Kouamé Mireille
  • Plateforme de Biologie Moléculaire, Pasteur Institute, Abidjan, Côte d’Ivoire, BP 490 Abidjan, Cote d’Ivoire.
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Aoussi Serge
  • Aoussi Serge
  • Plateforme de Biologie Moléculaire, Pasteur Institute, Abidjan, Côte d’Ivoire, BP 490 Abidjan, Cote d’Ivoire.
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Dozois Charles
  • Dozois Charles
  • Laboratoire de Microbiologie, Institut Armand Frappier, Laval, 531 Boul. des Prairies Laval (Québec), H7V 1B7, Canada.
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Dosso Mireille
  • Dosso Mireille
  • Plateforme de Biologie Moléculaire, Pasteur Institute, Abidjan, Côte d’Ivoire, BP 490 Abidjan, Cote d’Ivoire.
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  •  Received: 28 March 2020
  •  Accepted: 21 May 2020
  •  Published: 30 June 2020

Abstract

Bacteriophages (phages) are viruses that infect bacteria. The emergence of resistant bacteria has been reported worldwide. The use of phages to treat bacterial infection or to reduce bacteria load has become a new area of interest. The viral colonization of rodents represents an important source for a specific biotope. The objective of this study is to characterize the effect of novel phages isolated from rodents (Mastomys) using clinical Enterobacteria strains. Three novel phages were isolated from the intestinal tract of rodents. The phages belong to Myoviridae family. By using three Random Polymerase Chain Reactions (PCR), genomic characterization of the phages shows a high diversity that reveals several DNA bands. 3 virulent phages exhibited different patterns, indicating that the rodents’ phages are genetically unique and could be distinguished by typing-PCR. The virulence spectrum of the phages shows phages Mα3 and Mα7 have 50% (7/15) lytic activity on enteropathogenic and enterohemorrhagic Escherichia coli strains while phage Mα11 has 26.6% (4/15) lytic activity. These phages have no lytic activity on Salmonella strains. This study is the first to isolate novel phages from rodents with lytic activity. The candidate rodents’ phages (Mα3, Mα7, Mα11) can be used to control virulent E. coli in West Africa.

 

Keys words: Lytic phages, rodents, Escherichia coli strains, West Africa, enteropathogenic, enterohemorrhagic.