African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Thesis

Xylanase production using fruit waste as cost effective carbon source from thermo-tolerant Bacillus megaterium

Rafid Abdul Wahid Abdul Kareem
  • Rafid Abdul Wahid Abdul Kareem
  • Department of Biochemical Engineering, Alkhwarizmi College of Engineering, University of Baghdad, Baghdad, Iraq
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Imrana Khushk
  • Imrana Khushk
  • Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro, Pakistan
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Muhammad Aqeel Bhutto
  • Muhammad Aqeel Bhutto
  • Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro, Pakistan
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Qureshi Abdul Sattar
  • Qureshi Abdul Sattar
  • Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro, Pakistan
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Ayyaz Ahmed*
  • Ayyaz Ahmed*
  • Department of Chemical Engineering, University of Gujrat,Gujrat, Pakistan
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  •  Received: 12 December 2013
  •  Accepted: 17 March 2014
  •  Published: 17 September 2014

Abstract

Thermotolerant Bacillus megaterium BRL-0101 was used to produce xylanase in batch fermentations using various fruit waste as cost effective substrates. A mineral medium based on mango peel (100 g/L) as the carbon source and yeast extract + peptone + NaNO3 (10 g/L) as the nitrogen source proved to be the most effective. A 48-h batch fermentation in this medium with a starting pH of 8.0 produced a xylanase titer of ~2,876 U/mL at the optimal fermentation temperature of 50°C. The optimal temperature, initial pH, the carbon source and its concentration, and the nitrogen sources, size of inoculum, inducer and its concentration, were identified after evaluation of multiple nutrient sources and fermentation conditions.

Key words: Xylanase, Bacillus megaterium, fermentation, mango peel.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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