African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5230

Full Length Research Paper

Study of sputum and bronchoscopic lavage for acid fast bacilli in patients with pulmonary infections

  Azar Dokht Khosravi1,2*, Manigeh Mehdinejad1,2, Alireza Mozzafari3 and  Mohammad Hashemzadeh2  
  1Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran. 2Department. of Microbiology, School of Medicine, AJUMS, Ahvaz, Iran. 3Internal Medicine Ward, Golestan Teaching Hospital, AJUMS, Ahvaz, Iran.  
Email: [email protected]

  •  Accepted: 04 October 2010
  •  Published: 18 November 2010



The diagnosis of tuberculosis is based on the detection of Mycobacterium tuberculosis on clinical specimens with different methods. Although there are many diagnostic techniques, such as culture  and molecular methods, however, sputum smear microscopy for  demonstration of acid fast bacilli remains the most important diagnostic method for tuberculosis in high prevalence countries due to its speed, easy performance, and low cost. The aim of this study was determination of prevalence of acid fast bacilli (AFB) in specimens of suspected patients of pulmonary infections. In total, 2872 specimens of sputum and bronchoscopic lavage were collected. For smear preparation, the specimens were decontaminated and processed. Prepared smears were stained by Ziehl-Neelsen staining method as per standard guideline and examined under the light microscope for the presence of acid fast bacilli. From total specimens examined, 1726 (60%) were isolated from male patients and 1146 (40%) were from females. One hundred and eighty three (6.4%) were positive for acid fast bacilli. These were identified in 81.4% of sputum specimens and 18.4% of bronchoscopic lavages. The majority of smears were graded as 3+ according to criteria for AFB smear reporting. The results of the present study indicated that Ziehl Neelsen stain is preferable method for all suspected tuberculosis cases in absence of culture.


Key words: Sputum-bronchoscopic lavage, Ziehl Neelsen, acid fast bacilli, tuberculosis.