African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Antibacterial activity of two extracts from Rubus fruticosus L. against resistant pathogens and their antioxidant potential

AFIF CHAOUCHE Thanina*
  • AFIF CHAOUCHE Thanina*
  • Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE), Department of Biology, Faculty of Nature and Life, Earth and Universe Sciences, Aboubekr Belkaid University of Tlemcen, 13000 Tlemcen, Algeria
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BENDAHOU Mourad
  • BENDAHOU Mourad
  • Laboratory of Applied Microbiology in Food, Biomedical and Environment (LAMAABE), Department of Biology, Faculty of Nature and Life, Earth and Universe Sciences, Aboubekr Belkaid University of Tlemcen, 13000 Tlemcen, Algeria
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ARAB Karim
  • ARAB Karim
  • Laboratory of Valorization and Conservation of Biological Resources (VALCOR), Department of Biology, Faculty of Sciences, University of Boumerdes, 35000 Boumerdes, Algeria
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  •  Received: 18 February 2015
  •  Accepted: 27 April 2015
  •  Published: 06 May 2015

Abstract

One of the most serious threats to public health across the world is the spread of antibiotics resistant pathogens. This situation obliges researchers from all over the world to focus on how they can overcome this obstacle mainly by searching or synthesizing new molecules to neutralize microbes and to overcome the phenomenon of resistance. One of the main reservoirs of potentially active molecules is the plantae kingdom. In this work, we focused on Rubus fruticosus L., a medicinal species which is used traditionally in Algeria to heal respiratory infections. Both of chemical composition and biological activities of essential oils and flavonoids extract were explored. GC-MS analysis of essential oil reveals the Lanceol as the most abundant component (20.22%). HPLC applied on flavonoids extract shows the presence of the gallic acid, gallocatechin, protocatechuic acid, catechin, caffeic acid, rutin, ellagic acid and myricetin. Tests of antioxidant capacity using the DPPH reveals an inhibiting effect which is more important with the flavonoid extract. Otherwise, resistant strains to conventional antibiotics seem to be sensitive to the flavonoids of this species with MICs varying from 2.36 to 75.54 mg/ml. This study indicates that R. fruticosus L. has an important antimicrobial effect, which justifies its potential use in infectious diseases. For many reasons, this species remains poorly studied, further refined studies on its pure secondary metabolites are needed in the perspective of identifying new antimicrobial molecules from this plant.

Key words: Rubus fruticosus L., essential oil, flavonoids, antimicrobial activity, resistant respiratory pathogens, antioxidant activity.

Abbreviation

C.E, Crude extract; EC50, efficient concentration 50; DPPH, 2,2-diphenyl-1-picrylhydrazyl; MIC, minimal inhibitory concentration; Rt, retention time; HPLC, high performance liquid chromatography; GC-MS, gas chromatography-mass spectrophotometry; AMP, ampicilin; IMI, imipenem; GENT, gentamicin; AZITH, azithrimicin; CIP, ciproflaxin; VAN, vancomycin; OXA, oxacilin; TET, tetraciclin; ERYT, erythromicin; PENI, penicilin; AMO, amoxicilin; CEF, cefazolin; LEV, levoflaxin

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This article is published under the terms of the Creative Commons Attribution License 4.0

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