African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5215

Full Length Research Paper

Ciprofloxacin and tetracycline susceptibility of lactobacilli isolated from indigenous children’s feces

Yi-Chih Chang3,4, Yu-Chih Wang3, I-Kuan Wang5, Chuen-Fu Lin6, Hsiao-Li Chuang7, Yi-Ying Wu4, Tung-Ching Chung3* and Ter-Hsin Chen1,2*    
1The Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan. 2Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan. 3Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan. 4Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan. 5Division of Nephrology, China Medical University Hospital and College of Medicine, Taichung, Taiwan. 6Department of Medical Technology, Central Taiwan University of Science and Technology, Taichung, Taiwan. 7National Laboratory Animal Center, National Applied Research Laboratories, Taipei, Taiwan.
Email: [email protected].

  •  Accepted: 17 October 2011
  •  Published: 16 January 2012



To investigate the frequency of ciprofloxacin and tetracycline resistance lactobacilli in children feces, a total of 160 feces samples were cultured on Lactobacilli-selective Rogosa agar supplemented with 0.1 mg/ml of cycloheximide and 0.5% of CaCO3, and identified Lactobacillus species were identified by analysis of the PCR sequenced-16S rRNA gene through BLAST against the deposited GenBank database. In these samples, 96 isolates were obtained and identified as belonging to 6 species, including Lactobacilli plantarum, Lactobacilli helveticusi, Lactobacilli salivarius, Lactobacilli casei, Lactobacilli fermentum and Lactobacilli pentosus. Strain-subtyping of these isolates by repetitive extragenic palindromic (REP)-PCR demonstrated a notable genotypic biodiversity of 65.6%. Antimicrobial susceptibility of ciprofloxacin and tetracycline had a wide different minimum inhibitory concentration (MIC) values in these isolates. The MIC50 and MIC90 of ciprofloxacin both was 64 µg/ml for both, while the MIC50 and MIC90 of tetracycline were 128 and 512 µg/ml. These results indicate that high-level resistant activity of ciprofloxacin and tetracycline among Lactobacillus species in indigenous children’s intestines was prevalent in mountain district at the central area of Taiwan.

Key words: Indigenous child, ciprofloxacin and tetracycline resistance lactobacilli, Lactobacilli-selective rogosa agar, cycloheximide, antimicrobial susceptibility test, repetitive extragenic palindromic (REP)-PCR, minimum inhibitory concentration (MIC).