African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Restriction enzyme mediated integration and FIV lentiviral transgenesis applied to amphibians

Kaitlyn Hull
  • Kaitlyn Hull
  • Department of Poultry Science, Campus Box 7608, Raleigh NC 27695, North Carolina State University, USA.
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Dee Hodgson
  • Dee Hodgson
  • Department of Poultry Science, Campus Box 7608, Raleigh NC 27695, North Carolina State University, USA.
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James N. Petitte
  • James N. Petitte
  • Department of Poultry Science, Campus Box 7608, Raleigh NC 27695, North Carolina State University, USA.
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Bob Clark
  • Bob Clark
  • Nucleic Sciences, LLC. 6701 W. 121st Suite 200, Overland Park KS 66209, USA.
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Tim Hickok,
  • Tim Hickok,
  • Nucleic Sciences, LLC. 6701 W. 121st Suite 200, Overland Park KS 66209, USA.
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Lanette Fee
  • Lanette Fee
  • Department of Poultry Science, Campus Box 7608, Raleigh NC 27695, North Carolina State University, USA.
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Paul E. Mozdziak
  • Paul E. Mozdziak
  • Department of Poultry Science, Campus Box 7608, Raleigh NC 27695, North Carolina State University, USA.
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  •  Received: 01 March 2013
  •  Accepted: 28 October 2013
  •  Published: 01 January 2014

Abstract

Restriction enzyme mediated integration (REMI) transgenesis and lentiviral transgenesis are effective methods of introducing transgenes into the genome of frogs. One aquatic amphibian species, Xenopus laevis, and one land dwelling species, Litoria caerulea, were chosen as subjects for transgenesis. REMI produced X. laevis that expressed the fluorescent protein DsRed. REMI was unsuccessful in producing transgenic Litoria. Therefore, lentiviral transgenesis was attempted.  Hatchling Litoria tadpoles were exposed to replication defective lentiviral particles containing the coding sequence for DsRed. Histological evaluation revealed the presence of DsRed in brain, heart, liver, kidney, and muscle tissues. Therefore, lentiviral transgenesis appears to be a viable technique for producing transgenic land-dwelling frogs.

 

Key words: DsRed, Xenopus laevis, Litoria caerulea, restriction enzyme mediated integration (REMI), Lentivirus.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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