African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Genotypic variability for tolerance to salinity and phosphorus deficiency among N2-dependent recombinant inbred lines of Common Bean (Phaseolus vulgaris)

Boulbaba L’taief1,2,3,4,5*, Bouaziz Sifi2, Mainassara Zaman-Allah6, Ralf Horres3, Carlos Molina4, Steve Beebe7, Peter Winter3, Guenter Kahl4, Jean-acques Drevon5and Mokhtar Lachaâl1        
1Département de Biologie, Faculté de Sciences de Tunis, Campus universitaire, 2092 Tunis El Manar,Tunisie 1060, Tunisia. 2Laboratoire des grandes cultures, INRAT, Rue Hédi Karray 2080 Ariana, Tunisia. 3GenXPro GmbH, Frankfurt Innovation Center Biotechnology (FIZ), Altenhöferallee 3, 60438 Frankfurt am Main, Germany. 4Biocenter, University of Frankfurt/Main, 60439 Frankfurt am Main, Germany. 5INRA-IRD-SUPAGRO UMR1222 Ecologie Fonctionnelle et Biogéochimie des Sols, Place Viala,34060 Montpellier cedex 01, France. 6International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502324 AP, India. 7Centro Internacional de Agricultura Tropical, Cali, Colombia.
Email: [email protected]

  •  Accepted: 11 April 2011
  •  Published: 30 May 2012

Abstract

Common bean (Phaseolus vulgaris L.) is often subject to various environmental constraints including soil salinity and phosphorus deficiency as major limitations for the yield of most grain legumes, especially when the plant growth depends upon N2 fixation. In order to assess the genetic variation for tolerance to moderate salinity and phosphorus deficiency and identify the related morphological, physiological and genetic traits, 37 common beanrecombinant inbred lines (RILs) were inoculated with Rhizobium tropici CIAT899, and grown in a glasshouse with 25 mM NaCl or 75 µmol P plant-1 week-1, compared to optimal nutrient solution in hydroaeroponic culture system. Large genotypic variation in tolerance to P deficiency and salt was found with some RILs being tolerant to both constraints. By contrast some of the RILs showed tolerance to only one constraint while the most sensitive to salinity were also sensitive to P-deficiency. By using 18 microsatellite primer-pairs with six most contrasting RILs, 4 alleles were found to discriminate among the RILs. It is concluded that these genotypes and the microsatellites primers can be used to identify genes involved in salinity and P deficiency tolerance of N2-dependent legume.

 

Key words: Phaseolus vulgaris, genotypic variability, microsatellites, phosphorus deficiency, rhizobia; salinity, symbiosis.