African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Biofilm production, adherence and hydrophobicity of starved Shigella in seawater

Ali Ellafi*, Rihab Lagha, Fethi Ben Abdallah and Amina Bakhrouf      
Laboratory of Analysis, Treatment and Valorization of Environment Polluants and Product, Faculty of Pharmacy, Monastir University, Tunisia.  
Email: [email protected]

  •  Accepted: 27 December 2011
  •  Published: 30 May 2012


In this work, we incubated four Shigella strains for one month at room temperature and at 4°C. Their adhesion capacity to KB cells, biofilm formation on microplate and surface hydrophobicity of starved cells were investigated. Our results showed that the stressed cells exhibited a remarkable increase in their hydrophobicity from 19 to 34% and in their capacity to produce biofilm. Adherence assays to KB cells showed an increase in the number of adherent cells from 0.7 to 6.7% after one month of incubation in seawater. However, the percentage of invasion was decreased from 34 to 11% under stress condition.


Key words: Shigella, seawater, starvation, hydrophobicity, adhesion, invasion.