African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5056

Full Length Research Paper

Isolation of an emerging thermotolerant medically important Fungus, Lichtheimia ramosa from soil

Imade Yolanda Nsa
  • Imade Yolanda Nsa
  • Department of Microbiology, Faculty of Science, University of Lagos, Nigeria.
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Rukayat Odunayo Kareem
  • Rukayat Odunayo Kareem
  • Department of Microbiology, Faculty of Science, University of Lagos, Nigeria.
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Olubunmi Temitope Aina
  • Olubunmi Temitope Aina
  • Department of Microbiology, Faculty of Science, University of Lagos, Nigeria.
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Busayo Tosin Akinyemi
  • Busayo Tosin Akinyemi
  • Department of Microbiology, Faculty of Science, University of Lagos, Nigeria.
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  •  Received: 12 May 2020
  •  Accepted: 08 June 2020
  •  Published: 30 June 2020

Abstract

Lichtheimia ramosa, a ubiquitous clinically important mould was isolated during a screen for thermotolerant fungi obtained from soil on a freshly burnt field in Ikorodu, Lagos State. In the laboratory, as expected it grew more luxuriantly on Potato Dextrose Agar than on size limiting Rose Bengal Agar. The isolate had mycelia with a white cottony appearance on both media. It was then identified based on morphological appearance, microscopy and by fungal Internal Transcribed Spacer ITS-5.8S rDNA sequencing. This might be the first report of molecular identification of L. ramosa isolate from soil in Lagos, as previously documented information could not be obtained.
 
Key words: Soil, thermotolerant, Lichtheimia ramosa, Internal Transcribed Spacer (ITS).