African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5128

Full Length Research Paper

Evaluation of the currently used polymerase chain reaction assays for molecular detection of Brucella species

Moussa I. M.1, Omnia M. E.2, Amin A. S.2, Ashgan, M. H.3 and Selim S. A.4
  1Center of Excellence in Biotechnology Research, King Saud University, P. O. Box 2460, Riyadh 11451, Saudi Arabia. 2Biotechnology Research Unit, Animal Reproduction Research Institute, Egypt. 3College of Applied Studies and Community Service, King Saud University, Saudi Arabia. 4Microbiology Department, Faculty of Veterinary Medicine, Cairo University, Egypt.
Email: [email protected]

  •  Published: 18 June 2011

Abstract

 

The diagnosis of brucellosis is the corner stone in any control and eradication program. Therefore, the main objective of the present study was to apply more advanced techniques for rapid and accurate diagnosis of brucellosis that can overcome the draw backs of the traditional diagnostic techniques. Different polymerase chain reaction (PCR) assays were applied in the present study, either singly or in a multiplex format, that enable to detect and differentiate most of Brucella species. The PCR assay detection limit was evaluated in a preliminary study. The obtained results recommend the PCR assay as a valuable, rapid, very specific, highly sensitive and safe laboratory diagnostic test that can be used not only for detection of Brucella antigen either in culture or in clinical samples but also in differentiating most of the virulent and vaccinal strains.

 

Key words: Brucella species, polymerase chain reaction (PCR) assays, diagnosis, vaccinal strains, Brucella antigen.