African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5228

Full Length Research Paper

Investigation of accessory gene regulator (agr) in Staphylococcus aureus isolated from clinical and subclinical bovine mastitis in Iran

  Hassan Momtaz1*, Elahe Tajbakhsh2, Behnam Abbasian3 and Manochehr Momeni4
  1Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran. 2Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran. 3Department of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran. 4Department of Biotechnology, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
Email: [email protected]

  •  Accepted: 24 February 2010
  •  Published: 18 March 2010

Abstract

 

Mastitis is one of the common diseases of dairy cattle and an inflammatory response of the mammary glands tissue. Mastitis causes considerable loss to the dairy industry. Among several bacterial pathogens that can cause mastitis, S. aureus is probably the most lethal agent because it causes chronic and deep infection in the mammary glands that is extremely difficult to be cured. The present study was to detect agr group genes in the S. aureus isolated from 360 mastitis milk samples in Chaharmahel va Bakhtiari and Isfahan provinces of Iran via PCR by using specific primers. Among 360 milk samples, 86 samples contained 1250 bp fragment of the 23srRNA gene, 10 samples contained agrI gene, 42 samples contained agrII gene, 19 samples contained agrIII gene and 15 samples contained agrIV gene.

 

Key words:  Bovine mastitis, S. aureusagr group genes, PCR, Iran.