March 2010
An overview of pathogenesis and epidemiology of Helicobacter pylori infection
Helicobacter pylori induces chronic gastritis, the strongest known risk factor for peptic ulcer disease, distal gastric cancer and a number of extra gastric related morbidity. More than 50% of the world’s population is infected with this organism lifelong without effective bacterial eradication. Clinical sequelae are dependent upon bacterial virulence factors and host genetic diversity,...
March 2010
Advances in research of pathogenic mechanism of pine wilt disease
Pine wilt disease, caused by the pinewood nematode, Bursaphelenchus xylophilus, is the most serious disease of pine tree with great economic losses. So far it is not clear why the pine trees turn wilting, though several hypotheses about the pathogenicmechanism of pine wilt disease have been presented, such as phytotoxins causing death of pine trees; cellulases hydrolyzing celluloses of pine tree;...
March 2010
Separation and identification of lipase - producing strains and immobilized transesterification efficiency
12 strains of lipase - producing were isolated from 36 samples collected from oil - contaminated soil of machinery - repair plant, dining room, and vegetable market, respectively, of which strain (3 - 2) had the highest hydrolytic activity (17.1 U/ml). Immobilized cell of strain 3 - 2 onto sodium alginate and immobilized crude enzyme solution of strain (3 - 2) onto Sodium alginate were respectively used in...
March 2010
Study on b-galactosidase enzyme produced by isolated lactobacilli from milk and cheese
β-galactosidases enzyme have been used in the dairy industry for the improvement of lactose intolerance. The aim of this study was to detect b-galactosidase enzyme produced by isolated lactobacilli from milk and cheese. Isolated lactobacilli were cultured on MRS agar. Lactobacilli were identified by Gram stain and standard bacteriological and biochemical methods. Their ability to...
March 2010
An efficient protoplast isolation and regeneration system in Coprinus comatus
An efficient protocol for protoplast isolation and regeneration was first established inCoprinus Comatus. The highest yield of protoplasts was up to 8.9 × 106 cells/ml in digestion solution containing 2.0% lywallzyme and 0.6 M KCl after incubation of four-day-old mycelia at 30°C for 3 h; among which, about 1.4% protoplasts could be regenerated into mycelia after 4 - 6 days of...
March 2010
The antifungal susceptibilities of oral Candida spp isolates from HIV-infected patients
Oropharyngeal candidiasis (OPC) is the most common HIV related oral lesion. Most patients are infected with a strain originally present as a commensal of the oral cavity. The resistance of Candida isolates to antifungal drugs is important due to morbidity and mortality. The aim of our study was to investigate the antifungal susceptibility profiles of oral Candida spp which were...
March 2010
Investigation of accessory gene regulator (agr) in Staphylococcus aureus isolated from clinical and subclinical bovine mastitis in Iran
Mastitis is one of the common diseases of dairy cattle and an inflammatory response of the mammary glands tissue. Mastitis causes considerable loss to the dairy industry. Among several bacterial pathogens that can cause mastitis, S. aureus is probably the most lethal agent because it causes chronic and deep infection in the mammary glands that is extremely difficult to be cured. The present study...
March 2010
Irrigation influence by treated domestic wastewater instead of agronomical water on essential oil yield of basil (Ocimum basilicum L.)
In this experiment, we have used the soil profile as a biological filter that can to absorb pollution and microbes in the domestic wastewater at Iran during 2009. In this experiment, we had 15 lysimeters, that were planted canola (Brassica napus L.) in 1 to 5 lysimeters and were irrigated by domestic wastewater with BOD5 about 150 mg/lit and primary drainage...
March 2010
Adherence and damage to epithelial cells of human lung by Ureaplasma urealyticum strains biotype 1 and 2
Ureaplasma sp. is associated with chronic lung disease and it has been implicated in the morbidity and mortality of new born babies (human). Adherence is an important process for lung infection, and scarce information is available in this respect. This work shows its adhesion to epithelial cells from the respiratory tract in culture, and evaluates the Ureaplasma sp. references and clinical...
March 2010
Cloning and phylogenetic analysis of Bovine immunodeficiency virus env gene in Iranian isolates
Bovine immunodeficiency virus (BIV) is a member of the family Retroviridae, genusLentivirus that has three important gene including gag, pol and env. In the first step, PCR product of env gene of BIV isolated in different regions of Iran were cloned into a pTZ57R/T vector, then inserts were digested by BamHI and XhoI restriction enzymes and cloned into pGEX-4T-3 as an...
March 2010
A new alternative to treat swine influenza A virus infection: Extracts from Terminalia chebula Retz.
Currently, a pandemic swine influenza A virus infection causes a huge negative impact on human beings all over the world. However, the methods of treatment are not satisfactory, therefore it is urgent for us to set up new theory and practice to fight against the intractable virus. Terminalia chebula Retz., as a kind of traditional Chinese medicine, widely distributes and has multiple pharmacological...
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