Abstract

Pathogenic bacteria contaminate beverages and make consumers to have diarrhoeal diseases. This study was carried out to assess the occurrence and antimicrobial susceptibility of Escherichia coli and Salmonella spp. isolated from “zoom-koom” beverages and ice from open markets of Ouagadougou. One hundred samples of both “zoom-koom” and ice were analyzed using standard microbiological methods. Identification of serotypes of E. coli was completed using specific antisera. Strains were subjected to antimicrobial susceptibility test using agar diffusion method. E. coli was isolated in 36% “zoom-koom” and 35% ice samples. Among the 71 E. coli strains of both samples, there was 16.8% enteropathogenic E. coli (EPEC), belonging to six serotypes: E. coli O26, E. coli O55, E. coli O86, E. coli O119, E. coli O126 and E. coli O128. Salmonella was also found in 1% “zoom-koom” and 2% ice samples. Antibiotic susceptibility revealed that E. coli isolates resist amoxicillin-clavulanate, ticarcillin, cephalothin, cefamandole, ceftriaxone, cefepime, aztreonam, gentamicin, chloramphenicol, tetracycline, nalidixic acid and ciprofloxacin at different time. Meanwhile, Salmonella strains were sensitive to all tested antibiotics except tetracycline. The presence of EPEC and Salmonella spp. in the samples indicates that hygiene practices are being improved during processing to reduce the risk of having infection when they are consumed.

Keys words: “Zoom-koom”; ice, Escherichia coli, enteropathogenic E. coli (EPEC), Salmonella, antibiotics, Burkina Faso.