African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5182

Full Length Research Paper

Sensory, microbiological and physico-chemical characterization of Klila, a traditional cheese made in the south-west of Algeria

Benamara R. N.
  • Benamara R. N.
  • Laboratory of Microbiology Applied to Agribusiness, Biomedical and Environmental (LAMAABE), Faculty of SNV/STU, University of Tlemcen, 13000 Tlemcen, Algérie.
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Gemelas L.
  • Gemelas L.
  • Bioengineering and Microbial Dynamic at Food Interfaces (Associated team n°3733 (BioDyMIA), University of Lyon 1-ISARA Lyon), Isara-Lyon, Agrapole-23 rue Jean Baldassini, F-69364 Lyon Cedex 07, France.
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Ibri K.
  • Ibri K.
  • Departement of Agronomy, Faculty of Natural and Life Sciences, University of Mascara, BP 305 Pole Sidi Said, Mascara 29000, Algérie.
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Moussa-Boudjemaa B.
  • Moussa-Boudjemaa B.
  • Laboratory of Microbiology Applied to Agribusiness, Biomedical and Environmental (LAMAABE), Faculty of SNV/STU, University of Tlemcen, 13000 Tlemcen, Algérie.
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Demarigny Y.
  • Demarigny Y.
  • Bioengineering and Microbial Dynamic at Food Interfaces (Associated team n°3733 (BioDyMIA), University of Lyon 1-ISARA Lyon), Isara-Lyon, Agrapole-23 rue Jean Baldassini, F-69364 Lyon Cedex 07, France.
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  •  Received: 09 August 2016
  •  Accepted: 07 September 2016
  •  Published: 07 November 2016

Abstract

Klila, an Algerian cheese produced in steppe and mountainous areas, is proving increasingly popular with consumers. It is traditionally made with ewe, goat or cow milk, leading to a product with high dry matter content (> 90%). In this work, we have characterized three different Klila cheeses made with the three different milks using physico-chemical and microbiological parameters. A triangle test was also performed on naïve consumers, and the three types of Klila were clearly distinguished by sensory analysis. They exhibited distinct features, in particular very low Aw (< 0.5) and pH values (< 4.5) preserving them from pathogens. Lactobacilli and enterococci were counted at low levels (< 4 log (cfu)/g) as well as some spore-forming bacteria (< 3 log (cfu)/g). Colonies were picked from MRS and BEA media. They were identified by sequencing and characterized on their ability to produce lactic acid and using REP-PCR. Lb plantarum was the main species isolated, followed by Pediococcus pentosaceus, Leuconostoc pseudomesenteroïdes and Lactobacillus fermentum. The Enterococcus genus was dominated by Ec durans, Ec faecium and Ec hirae. Among these two main populations, different subgroups were observed by means of the REP-PCR profiles and the lactic acid production of the isolates. Some strains were found in two and even three cheeses. We suppose that these microbes are representative of the environmental context in which Klila is produced.

Key words: Klila, lactic acid bacteria, Algerian traditional cheese, Lactobacillus plantarum, Enterococcus.