African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4982

Full Length Research Paper

The bacterial diversity in infected tissue pus of an East Asian finless porpoise

Caiwen Wu
  • Caiwen Wu
  • Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Wenyuan Road 1, Qixia District, Nanjing 210023, China.
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Zhe Hong
  • Zhe Hong
  • College of Life Science, Shenyang Normal University, Huanghebei Street 253, Shenyang, 110034, China.
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Aamir Ibrahim
  • Aamir Ibrahim
  • Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Wenyuan Road 1, Qixia District, Nanjing 210023, China.
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Di Zhang
  • Di Zhang
  • Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Wenyuan Road 1, Qixia District, Nanjing 210023, China.
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Lin Wang
  • Lin Wang
  • Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Wenyuan Road 1, Qixia District, Nanjing 210023, China.
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Ting Li
  • Ting Li
  • Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Wenyuan Road 1, Qixia District, Nanjing 210023, China.
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Bingyao Chen
  • Bingyao Chen
  • Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Wenyuan Road 1, Qixia District, Nanjing 210023, China.
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  •  Received: 22 September 2019
  •  Accepted: 30 October 2019
  •  Published: 30 November 2019

Abstract

Two East Asian finless porpoises (Neophocaena asiaeorientalis sunameri) in Ningbo, East China Sea, China, were observed to be bacterially infected between the fat layer and muscle layer. The microbial communities in pus samples were identified by metagenome sequencing 16S in the PacBio platform and explored the relationship between emaciation and bacterial infection. The present paper was the first report on bacterial diversity in infected tissue pus of finless porpoise. In total, 101 bacterial species were identified, and the top nine species were Rhodococcus qingshengii (26.25%), Rhodococcus jialingiae (22.43%), Ralstonia pickettii (16.03%), Moraxella osloensis (9.97%), Psychrobacter cibarius (2.97%), Flavobacterium johnsoniae (2.11%), Flavobacterium chungbukense (1.80%), Stenotrophomonas maltophilia (1.73%) and Serratia marcescens (1.62%). These main bacteria could cause various diseases or metabolic disorder, such as endocarditis, meningitis, bacteraemia, septicaemia, and so on; they also involve microbiome metabolism of amino acids (12.47%), carbohydrates (11.58%), xenobiotic biodegradation (7.81%), lipids (6.01%) and energy (4.98%). Regardless of the type of disease metabolic disorder will firstly cause body weight loss. In conclusion, these bacteria could cause diseases or metabolic disorders, resulting in emaciation of East Asian finless porpoises. Admittedly, pathogenic assay is furthermore needed to determine the mechanisms regarding the pathological phenomena.

 

Key words: Bacterial diversity, East Asian finless porpoises, Metagenome sequencing 16S.