African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Genetic variation among isolates of Rigidoporus microporus causing white root disease of rubber trees in Southern Thailand revealed by ISSR markers and pathogenicity

Saithong Kaewchai1*, Hong Kai Wang2, Fu-Cheng Lin2, Kevin D Hyde3 and Kasem Soytong4
1Biotechnology in Plant Pathology, International College, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand. 2College of Agriculture and Biotechnology, Biotechnology Institute, Zhejiang University, Hangzhou, P. R. China. 3School of Science, Mae Fah Luang University, Chiang Rai, Thailand. 4Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand.
Email: [email protected]

  •  Accepted: 27 August 2009
  •  Published: 31 October 2009

Abstract

The genetic variation among 32 isolates of Rigidoporus microporus was determined using pathogenicity tests and molecular marker (intersimple sequence repeats; ISSR). Isolates were collected from six sites of two provinces in the south of Thailand. Based on the pathogenicity results, all isolates could divide into three groups depended on their virulence to rubber trees as follows: low virulent isolates, moderately virulent isolates and high virulent isolates. Cluster analysis based on ISSR characters grouped the isolates according to geographical origins. A dendrogram resulting from a cluster analysis showed two main distinct groups, designated as A and B rooting from outgroup. Group A contained isolates collected from Surat Thani province and group B those collected from Narathiwat province. There was no clear relationship between degrees of disease and geographical regions.

 

Key words: ISSR, pathogenicity, Rigidoporus microporus, white root disease.