October 2009
High infection activities of two Esteya vermicola isolates against pinewood nematode
Esteya vermicola, as the first recorded endoparasitic fungus of pinewood nematode, exhibits high infection activity and shows potential as a biological control agent to combat the devastating pine wilt disease. However, there is still a paucity of data about this rare hyphomycete. In this article, two E. vermicola isolates, CBS 100821 and CBS 115803, were studied in the morphological...
October 2009
Colour removal of textile dyes by culture extracts obtained from white rot fungi
In this study, the decolourizations of 36 different textile dyestuffs were studied with 6 different white rot fungi by solid state fermentation. Extra cellular enzymes secreted by fungi and total protein analyses were carried out in culture extracts obtained, according to the enzyme analyses, the highest activity was found in Laetiporus sulphurous and Coriolus versicolor. In our experiments involving...
October 2009
Antimicrobial resistance pattern among intensive care unit patients
Nosocomial Infections (NIs) are an important cause of morbidity, mortality and economic problems especially in intensive care units (ICUs). This study aim was to investigate antimicrobial resistance pattern of the regional microorganisms in ICU patients. In a cross sectional study, 500 specimens from patients admitted in the ICU who had signs or symptoms of nosocomial infection were collected (2005 - 2006). For each...
October 2009
Effects of Verticillium dahliae Kleb., Fusarium oxysporum Schlecht. f. sp. tuberosi Snyder, Hansen and Meloidogyne javanica (Treub.) Chitwood inoculated individually or in combination on potato growth, wilt severity and nematode development
The present study showed the potential risk of coexistence of Verticillium dahliae,Fusarium oxysporum f. sp. tuberosi and Meloidogyne javanica, according to fungal isolates present, on potato culture and the severity of fungal and nematological attacks. A synergistic interaction within agents of the parasitic complex studied was expressed by a reduction in plant growth, an increase in vascular...
October 2009
Glutaminase activity of Lactobacillus reuteri KCTC3594 and expression of the activity in other Lactobacillus spp. by introduction of the glutaminase gene
We investigated the properties of glutaminase activity of Lactobacillus reuteriKCTC3594 and expressed the glutaminase gene (EC 3.5.1.2) heterologously. The enzyme activity was optimal at pH 7.5 and 40°C. High salt–tolerance of the enzyme was observed as 50% of the initial activity although it still remained at 20% (w/v) NaCl for 30 min. The glutaminase gene was cloned from L. reuteri KCTC3594 by...
October 2009
Deletion of Pol IA alleles increases sensitivity to hydrogen peroxide in the parasitic protozoan Trypanosoma brucei
Trypanosoma brucei is the etiological agent of African Trypanosomiasis. T. bruceipossesses biologically unique mitochondrial DNA called kinetoplast DNA (kDNA). Genetic studies provide strong evidence that kDNA is essential. Understanding kDNA maintenance may yield promising drug targets. RNA interference (RNAi) studies of mitochrondrial DNA polymerase IA (POL IA) did not show a growth phenotype...
October 2009
Biodegradation of mononucleotides to nucleosides and free bases
An intracellular phosphate, non repressible alkaline phosphate found in extracts ofPenicillium brevicompactum NRC829 could catalyze dephosphorylation of ribonucleotides AMP, GMP, CMP, UMP and phenyl disodium orthophosphate (phph) optimally at pH 9.0 and 60°C. The extracts contain also hydrolytic deamination activities with adenosine, cytidine and cytosine out of the tested ribonucleotides, ribonucleosides and...
October 2009
Prevalence and distribution of Vibrio vulnificus in fishes caught off Chennai, Indian ocean
Vibrio vulnificus is a notorious sea-food borne pathogen with a high mortality rate. This is ubiquitously present in marine environments, particularly in tropical water. In these studies the prevalence of V. vulnificus in fishes caught off Chennai coast of Indian Ocean are determined. Commercially important fishes were analyzed for the occurrence of vibrios of which some of them were harbored fishes....
October 2009
In vitro microbial efficacy analysis of Supime, a fixed dose combination of cefepime and sulbactam, in comparison with cefepime alone
Cefepime is a fourth generation cephalosporin having an extended spectrum of activity against gram positive and gram negative bacteria. Sulbactam is a β-lactamase inhibitor similar in structure to clavulanic acid. In presence of sufficient β-lactamase inhibitor, the β-lactamase enzymes are neutralized and thus the drug used in combination with inhibitor has an opportunity to be more bactericidal and is of...
October 2009
Comparison of RNA extraction methods applied to gene cloning of the taxol-producing fungi
The qualities of RNA are an important foundation for molecular biology research. In this study, five different methods were selected for extracting RNA from the taxol-producing endophytic fungi. Yield and quality of isolated RNA were assessed by routine test method. The results showed that the Trizol method perform was better than others method in the yield, but the LiCl method perform better than others methods in...
October 2009
Cell cultures contaminations by mycoplasmas
The contamination of cell cultures with mycoplasmas can have different cytogenetic effects, usually adhere to cell but, depending on the species, these bacteria deplete the nutrients of cell cultures and interfere with the response of these cells when challenged experimentally. The objective of the present study was mycoplasmas detection in cell cultures came from biomedical laboratories. The cell cultures were screened...
October 2009
Genetic variation among isolates of Rigidoporus microporus causing white root disease of rubber trees in Southern Thailand revealed by ISSR markers and pathogenicity
The genetic variation among 32 isolates of Rigidoporus microporus was determined using pathogenicity tests and molecular marker (intersimple sequence repeats; ISSR). Isolates were collected from six sites of two provinces in the south of Thailand. Based on the pathogenicity results, all isolates could divide into three groups depended on their virulence to rubber trees as follows: low virulent isolates,...
October 2009
Decontamination of ready-to-eat Japanese mustard green (Brassica japonica) from Escherichia coli using slightly acidic electrolyzed water
In the present study, slightly acidic electrolyzed water (SAEW: pH 5.6 ± 0.21, 22 ± 1.4 mg/L available chlorine concentration; ACC) was evaluated for decontamination of Japanese mustard green (Brassica japonica) from Escherichia coli (E. coli) and its effectiveness was compared to that of sodium hypochlorite solution (NaOCl solution: pH 9.8±0.01, 110 ± 2.3 mg/L ACC)....
October 2009
Adenosine deaminase level in the serum of the patients Toxoplasma gondii seropositive and Giardia intestinalis
Adenosine deaminase (ADA) is an aminohydrolase making adenosine, deoksiadenozini inozin, and deocsiniozine deaminise irreversibly and plays role in the catabolism of purine nucleotids. Toxoplasma gondii is a zoonoses intracellular parasite that causes infection in animals and humans. This parasite encompasses enzymes that produce free radicals such as superoxide and hydrogen peroxide. In addition, Giardia...
October 2009
Isolation of Fusarium sp. AF4 from sewage sludge, with the ability to adhere the surface of polyethylene
Plastic materials like Polyethylene (PE) are the potential source of environmental pollution. Their presence in soil causes infertility of soil, preventing degradation of other normal substances, in addition to which it presents a danger to animal life. In the present study, a fungal strain was isolated from sewage sludge capable of adhering to the surface of PE pieces, through enrichment technique. A thick network of...
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