African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5173

Full Length Research Paper

Molecular detection of invA, ssaP, sseC and pipB genes in Salmonella typhimurium isolated from human and poultry in Iran

Ali Amini Fazl1, Taghi Zahraei Salehi1*, Mahmood Jamshidian1, Kumarss Amini2 and Amir Hossein Jangjou1
1Department of Microbiology, Faculty of Specialized Veterinary Science, Islamic Azad University, Science and Research Branch, Tehran, Iran. 2Department of Microbiology, Faculty of Agriculture, Islamic Azad University, Saveh Branch, Saveh, Iran
Email: [email protected]

  •  Accepted: 08 March 2013
  •  Published: 26 March 2013

Abstract

Infections due to Salmonella serovars are a great risk to public health. It is known that genes involved in pathogenesis of Salmonella serovars are clustered within Salmonellapathogenicity Islands. We have characterized four genes (invAssaPsseC, and pipB) coded by 3 pathogenicity Islands among 54 Salmonella typhimurium isolated from poultry samples in Iran. A set of tetraplex polymerase chain reaction (PCR) was developed. Results show that invA, ssaP, sseC, and pipB genes were 100% prevalent in this study. This assay is a reproducible and sensitive assay for the detection of Salmonella by screening several virulent genes present in SPIs. Because of rapidity, high specificity and sensitivity of M-PCR method, by standardization of this method, it could be considered as an alternative to conventional culture and serotyping methods to confirm the presence ofSalmonella in human and poultry samples. This is the first study on the distribution ofssaP, sseC and pipB genes in isolates from human and poultry sources in Iran.

 

Key words: Salmonella typhimurium, multiplex PCR, invAssaPsseCpipB, gene.