African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Strain improvement in Pleurotus Ostreatus using UV light and ethyl methyl sulfonate as mutagens

Rishu Sharma*
  • Rishu Sharma*
  • Department of Plant Pathology, Centre for Mushroom Research and Training, CSKHPKV, Palampur, Himachal Pradesh, 176062, India.
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B. M. Sharma
  • B. M. Sharma
  • Department of Plant Pathology, Centre for Mushroom Research and Training, CSKHPKV, Palampur, Himachal Pradesh, 176062, India.
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  •  Accepted: 13 January 2014
  •  Published: 29 January 2014

Abstract

Oyster mushroom (Pleurotus Ostreatus) is the choicest edible species cultivated in various regions of the world. Strain improvement studies were carried out in three strains of P. Ostreatus spp. Three strains of P. Ostreatus viz. PO-2, PO-6 and PO-7 were used for strain improvement, emphasizing on lower spore count and colour of the sporophore. It is a gymnocarpous genus of mushroom, which continuously release spores in its close vicinity causing various respiratory allergies. Their spores are highly potent allergens which can also cause exogenous allergic alveolitis. Attempts were made to produce low sporing strains of P. Ostreatus through mutagenesis using physical mutagen (UV light) and chemical mutagen (ethyl methyl sulfonate, EMS). Spores of three strains of P. Ostreatus spp. were given different treatments with UV light and EMS. Mutants exhibited appressed mycelial growth and showed slower spawn run and creamish white sporophore in PO 7(U4).  A lower spore count was also observed in PO-7(E3) mutant as compared to control.

Key words: Pleurotus, UV light, ethyl methyl sulfonate, mutagens.