African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

An efficient protoplast isolation and regeneration system in Coprinus comatus

Linhui Li1*, Qingyu Yin1, Xiaohong Liu1 and Hong Yang2
  1Key Laboratory of Southwest China Wildlife Resources Conservation, China West Normal University, Nanchong City 637009, P. R. China. 2Department of Biochemistry, Xichang College, Xichang City 615022, P. R. China.

  •  Accepted: 24 February 2010
  •  Published: 18 March 2010

Abstract

 

An efficient protocol for protoplast isolation and regeneration was first established inCoprinus Comatus. The highest yield of protoplasts was up to 8.9 × 106 cells/ml in digestion solution containing 2.0% lywallzyme and 0.6 M KCl after incubation of four-day-old mycelia at 30°C for 3 h; among which, about 1.4% protoplasts could be regenerated into mycelia after 4 - 6 days of incubation at 25°C in CYM medium with0.6 M mannitol as osmotic stabilizer. The results are beneficial for breeding new cultivars by the methods such as protoplast fusion, mutagenesis as well as transformation. Moreover, the stepwise procedure for protoplast liberation and regeneration could be referred in other species.

 

Key words: Coprinus comatus, mycelia, lywallzyme, protoplast, liberation and regeneration.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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