Staphylococcus aureus is Gram positive cocci which causes different infections, some of these strains are resistant to Methicillin (MRSA) and can be found in hospitals. S. aureus is one cause of nosocomial infections. The aim of this study was to find MRSA and Vancomycin resistant S. aureus (VRSA) in Fatemeh Zahra Hospital. Clinical samples were isolated from confined patients in the hospital were cultured on blood agar and mannitol salt agar and the positive coagulase and catalase colonies which were able to ferment the mannitol and trehalose were considered as S. aureus. These organisms were antibiogramed by disc diffusion method and E. test. In this research 72 species of S. aureuswere collected from various clinical samples in hospital, the rate of resistance to different antibiotics is as follow: oxacillin, 59.72%; ciprofloxacin, 22.22%; Ceftizoxime, 51.38%; co-trimoxazole, 38.88%; tetracycline, 52.77% and all the strains were sensitive to vancomycin. In this study prevalence of oxacillin or methicillin resistant was 59.72% and vancomycin resistance or intermediate S. aureus was not detected. Treatment based on antibiogram test results and rational antibiotics therapy can prevent increase in the number of resistant species.

Key words: Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA), methicillin, vancomycin.