African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5188

Full Length Research Paper

Prevalence of Listeria species and Listeria monocytogenes serotypes in ready mayonnaise salads and salad vegetables in Iran

Hossein Jamali1,2*, Mohammadjavad Paydar2,3 , Chung Yeng Looi3 and Won Fen Wong4
1Microbiology Division, Insititute of Biological Science, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia. 2Laboratory of Biomedical Science and Molecular Microbiology, Institute of Graduate Studies, University of Malaya, 50603, Kuala Lumpur, Malaysia. 3Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603 Malaysia. 4Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603 Malaysia
Email: [email protected]

  •  Accepted: 26 April 2013
  •  Published: 07 May 2013

Abstract

Three hundred samples of ready mayonnaise salads and salad vegetables were used forisolation of Listeria spp. by ISO 11290-1. The isolates were identified using biochemical tests, further confirmed by duplex PCR and differentiated via conventional agglutination serotyping. A total of 8.7% of the samples harboured Listeria spp., including 7% Listeriamonocytogenes, 1% Listeria innocua and 0.7% Listeria welshimeri. The L. monocytogenes isolates were divided into four serotypes of 1/2a (61.9%), 1/2b (19%), 3b (4.8%) and 4b (14.3%). The prevalence of L. monocytogenes, especially serotype 4b indicates that ready mayonnaise salads and salad vegetables could be potent sources of listeriosis.

 

Key words: Listeria spp., Listeria monocytogenes, serotyping, ready mayonnaise salads, salad vegetables.