African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Identification of Edwardsiella tarda isolated from duck and virulence genes detection

Xuepeng Wang1, Maocang Yan2*, Qishuo Wang3, Lei Ding1 and Fuchang Li1
1College of Animal Science and Technology, Shandong Agricultural University, Tai’an, 271018, P.R. China. 2Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Wenzhou, 325005, P.R. China. 3Huai’an Research Center, Institute of Hydrobiology, Chinese Academy of Sciences, Huai’an 223002, P.R. China.
Email: [email protected]

  •  Accepted: 23 April 2012
  •  Published: 21 June 2012

Abstract

Bacteria from 30 duck liver samples were characterized using VITEK System. Two strains showed 99% similarity to Edwardsiella tarda (E. tarda). A set of seven virulence genes viz.citC (citrate lyaseligase), fimA (fimbrial operon), gadB (glutamate decarboxylase), katB(catalase), mukF (putative killing factor), esrB (TTSS regulator) and gyrB (ATPase domain of DNA gyrase) were amplified from obtained duck E. tarda and reference fish E. tarda. The results obtained show that all seven genes were present in the isolate X1 and reference strain, while only gyrB, citC and mukF genes were present in the isolate X2. The results obtained also indicate that X1, X2, and reference strain were all virulence strains.

 

Key words: Duck, E. tarda, Identification, virulence genes.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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