African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5228

Full Length Research Paper

Glutaminase activity of Lactobacillus reuteri KCTC3594 and expression of the activity in other Lactobacillus spp. by introduction of the glutaminase gene

Jeong-Min Jeon1, Hae-In Lee1 and Jae-Seong So1,2*
1Department of Marine Science and Biotechnology BK21 Program, Inha University, Incheon 402-751, South Korea. 2Department of Biological Engineering, Inha University, Incheon 402-751, South Korea.
Email: [email protected]

  •  Accepted: 21 August 2009
  •  Published: 31 October 2009

Abstract

We investigated the properties of glutaminase activity of Lactobacillus reuteriKCTC3594 and expressed the glutaminase gene (EC 3.5.1.2) heterologously. The enzyme activity was optimal at pH 7.5 and 40°C. High salt–tolerance of the enzyme was observed as 50% of the initial activity although it still remained at 20% (w/v) NaCl for 30 min. The glutaminase gene was cloned from L. reuteri KCTC3594 by PCR, and subsequently introduced into two Korean isolates of Lactobacillus species. All of the transformants harboring the glutaminase gene from L. reuteri KCTC3594 were able to elevate glutaminase activity when introduced into other lactobacilli.

 

Key words: Lactobacillus spp., glutaminase, salt-tolerance, natural flavor, glutaminase gene, heteroexpression.

Abbreviation

MSG; Monosodium glutamate, KGA; Kidney (K-type) glutaminase, LGA; Liver (L-type) isoenzyme.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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