African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5169

Full Length Research Paper

Occurrence of Klebsiella oxytoca producing extended-spectrum beta-lactamases in different seasons in Ilam Hospitals, Iran

Sobhan Ghafouryan1, Nourkhoda Sadeghifard2, VasanthaKumari Neela1, Nor Shamsudin Mariana1, Reza Mohebi2, Mohammad Rahbar3 , Ali Hematian2, Zamberi bin Sekawi1*, Mohammad Raftari 4 and Iraj Pakzad2 
  1Department of Medical Microbiology, and Parasitology, Faculty of Medicine and Science Health, University Putra Malaysia 2Department of Medical Microbiology, Ilam university of Medical Sciences, Iran. 3Reference laboratory of Iran. 4Faculty of Food Science and Technology, University Putra Malaysia, Malaysia  
Email: [email protected]

  •  Accepted: 23 September 2010
  •  Published: 18 November 2010

Abstract

Our study focused on assessing frequency of Klebsiella oxytoca, to study incidence ofK. oxytoca producing ESBLs, to investigate frequency of blaTEM, blaSHV and blaCTX-M and to determine resistance of K. oxytoca producing ESBLs toward non-beta-lactam antibiotics, in different seasons in Ilam hospital. Twelve clinical isolates of K. oxytoca were found during March 2007 to April 2008 in Ilam hospital in Ilam city in west of Iran. The strains were isolated from admitted patients in surgery wards, lesion and respiratory tract infection. ESBLs identified by phenotypic and genotypic methods. PCR had done for detection of blaSHV, TEM and CTX-M. K. oxytoca producing ESBLS were evaluated against non-beta-lactam antibiotics of twelve K. oxytoca collected in Ilam hospitals, 16.67% (n=2), 16.67% (n=2) and 66.66% (n=8) were from the surgery wards, lesion, and respiratory tract infections (RTIs), respectively. 25% of K. oxytocawere ESBLs positive. blaSHV were found as responsible for ESBLs production. All theK. oxytoca producing ESBLs were susceptible to non-beta-lactam antibiotics. The highest frequency of K. oxytoca were found from patients with RTI (66.7%) and the lowest frequency of K. oxytoca had observed in admitted patients in surgery ward and patients with lesion infections, as an equal (16.6%). Our finding showed that resistant to ceftazidime was more than the others antibiotics (41.66%). We were found the most ESBLs production occurred in winter in K. oxytoca isolated in surgery ward (50%).

 

Key word: K. oxytoca, ESBLs, Ilam Hospitals, Iran..