African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5044

Full Length Research Paper

Antibiotic resistance and genotypic detection of extended spectrum beta-lactamase producing pathogens in catheter associated urinary tract infection at a teaching facility in Kumasi, Ghana

Alexander Bekoe
  • Alexander Bekoe
  • Department of Theoretical and Applied Biology, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
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Roland Azorliade
  • Roland Azorliade
  • Department of Urology, Komfo Anokye Teaching Hospital, Kumasi, Ghana.
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Anthony Ablordey
  • Anthony Ablordey
  • Department of Bacteriology, Noguchi Memory Institute for Medical Research, University of Ghana, Legon, Ghana.
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Matthew Glover Addo
  • Matthew Glover Addo
  • Department of Theoretical and Applied Biology, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
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  •  Received: 12 June 2020
  •  Accepted: 23 July 2020
  •  Published: 31 August 2020

Abstract

This study determines the antibiotic resistance pattern of extended spectrum beta-lactamase (ESBL) producing pathogens responsible for catheter associated urinary tract infection (CAUTI) and the genes associated with the resistance. The study used 35 ESBL-producing pathogens isolated from urine and biofilm found in CAUTI from June 2018 to November 2018. Pathogens were confirmed phenotypically for ESBL production with cefpodoxime combination disc kits. Antibiotic resistance was tested using the Kirby-Baurer disc diffusion method on Muller Hinton Agar. ESBL genotypes were identified with PCR. Urine isolates showed higher frequency of resistance against ciprofloxacin (94.11%), cefuroxime and ceftazidime (82.35%) and with no recorded resistance against Eertepenem (0.0%). The average resistance of the biofilm isolates ranged from 0.0% (Ertepenem) to 88.89% (Cefuroxime, Cefpodoxime, Ciprofloxacin and Trimethoprim). All the targeted genes were identified with CTX-M (40%) being the most dominant among them. The ESBL-producing pathogens showed zero resistance against Ertepenem. Ciprofloxacin and other Cephems commonly used in CAUTI treatment were shown to be less effective. The high resistance is as a result of the bacterial cells present in the biofilm with Klebsiella pneumonia exhibiting more resistance than the ESBL-producing E. coli and CTX-M-1 was identified as the most prevalent gene among the identified genotypes. Ertepenem should therefore be recommended for treatment of Catheter associated urinary tract infection.

 

Key words: Catheter, CAUTI, extended spectrum beta-lactamase (ESBL), urine, biofilm, resistance, microorganisms.