African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Optimization of fermentation conditions for the anti-cyanobacterial substances production by Streptomyces sp. HJC-D1 using response surface methodology

Yun Kong*
  • Yun Kong*
  • 1. Department of Environmental Engineering, Zhejiang University, Zhejiang Hangzhou 310058, China; 3. Yixing Urban Supervision and Inspection Administration of Product Quality, National Supervision and Inspection Center of Environmental Protection Equipment Quality (Jiangsu, preparation), Jiangsu Yixing 214205, China.
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Pei Zou
  • Pei Zou
  • Zhongyi Instrument Co., Ltd., Jiangsu Yixing 214205, China
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Jiaoqin Qi
  • Jiaoqin Qi
  • 1. Department of Environmental Engineering, Zhejiang University, Zhejiang Hangzhou 310058, China; 3. Yixing Urban Supervision and Inspection Administration of Product Quality, National Supervision and Inspection Center of Environmental Protection Equipment Quality (Jiangsu, preparation), Jiangsu Yixing 214205, China.
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Liming Song
  • Liming Song
  • Yixing Urban Supervision and Inspection Administration of Product Quality, National Supervision and Inspection Center of Environmental Protection Equipment Quality (Jiangsu, preparation), Jiangsu Yixing 214205, China
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Fei Shao
  • Fei Shao
  • Yixing Urban Supervision and Inspection Administration of Product Quality, National Supervision and Inspection Center of Environmental Protection Equipment Quality (Jiangsu, preparation), Jiangsu Yixing 214205, China
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An Zheng
  • An Zheng
  • Yixing Urban Supervision and Inspection Administration of Product Quality, National Supervision and Inspection Center of Environmental Protection Equipment Quality (Jiangsu, preparation), Jiangsu Yixing 214205, China
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Chengcheng Xie
  • Chengcheng Xie
  • Yixing Industrial Park for Environmental Science and Technology, Jiangsu Yixing 214205, China
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Jianzhong Zhou
  • Jianzhong Zhou
  • Yixing Industrial Park for Environmental Science and Technology, Jiangsu Yixing 214205, China
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Liang Zhu
  • Liang Zhu
  • Department of Environmental Engineering, Zhejiang University, Zhejiang Hangzhou 310058, China
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Xiangyang Xu
  • Xiangyang Xu
  • Department of Environmental Engineering, Zhejiang University, Zhejiang Hangzhou 310058, China
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  •  Received: 25 October 2012
  •  Published: 12 November 2014

Abstract

To investigate the influence of fermentation conditions such as temperature, initial pH, volume and agitation rate on anti-cyanobacterial active substances production, response surface methodology (RSM) was carried out to optimize the fermentation conditions of an anti-cyanobacterium Streptomyces sp. HJC-D1, and the anti-cyanobacterial effect was evaluated. Most common and widespread bloom-forming cyanobacterium Microcystis aeruginosa that is associated with microcystic toxins secretion was used as indicator cyanobacterium. The central composite design (CCD) was applied to evaluate the combined effects of the four factors, that is, temperature, initial pH, volume and agitation rate. Based on the analysis of 30 performed experiments, the best optimum level of operating parameters was 33.1°C for temperature, 11.8 for initial pH, 91.2 mL for volume and 337.5 rpm for agitation rate. Additionally, the maximum removal efficiency of chlorophyll a under the optimized culture conditions in flask cultures was 93.7%. It is noteworthy that the yield of the anti-cyanobacterial active substances produced by Streptomyces sp. HJC-D1 was significantly improved using response surface methodology and suggested the potential to develop a commercial biological control agent against M. aeruginosa.

Key words: Response surface methodology, optimization, fermentation conditions, anti-cyanobacterial effect, eutrophication control.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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